Enhancing the performance of an in vitro RNA biosensor through iterative design of experiments
Sign Up to like & getrecommendations! Published in 2025 at "Biotechnology Progress"
DOI: 10.1002/btpr.70005
Abstract: The quality control of RNA has become increasingly crucial with the rise of mRNA‐based vaccines and therapeutics. However, conventional methods such as LC–MS often require specialized equipment and expertise, limiting their applicability to high throughput… read more here.
Keywords: rna; design experiments; enhancing performance; biosensor ... See more keywords
Design of experiments as a valuable tool for biopharmaceutical analysis with (imaged) capillary isoelectric focusing
Sign Up to like & getrecommendations! Published in 2019 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201900162
Abstract: Capillary isoelectric focusing is indispensable for characterizing charge heterogeneity and isoelectric points of biopharmaceuticals. However, there are many influencing parameters and therefore method development is challenging. This study was performed to obtain an in‐depth understanding… read more here.
Keywords: capillary isoelectric; experiments valuable; valuable tool; design experiments ... See more keywords
Design of experiments‐based high‐throughput strategy for development and optimization of efficient cell disruption protocols
Sign Up to like & getrecommendations! Published in 2017 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.201600030
Abstract: Efficient and reproducible cell lysis is a crucial step during downstream processing of intracellular products. The composition of an optimal lysis buffer should be chosen depending on the organism, its growth status, the applied detection… read more here.
Keywords: design experiments; lysis buffer; lysis; high throughput ... See more keywords
Efficient Optimization of Process Strategies with Model-Assisted Design of Experiments.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0191-4_13
Abstract: Conventional design of experiments (DoE) methods require expert knowledge about the investigated factors and their boundary values and mostly lead to multiple rounds of time-consuming and costly experiments. The combination of DoE with mathematical process… read more here.
Keywords: design experiments; process; efficient optimization; model assisted ... See more keywords
Using a Design of Experiments Approach to Inform the Design of Hybrid Synthetic Yeast Promoters.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0822-7_1
Abstract: Hybrid promoter engineering takes advantage of the modular nature of eukaryotic promoters by combining discrete promoter motifs to confer novel regulatory function. By combinatorially screening sequence libraries for trans-acting transcriptional operators, activators, repressors and core promoter… read more here.
Keywords: using design; experiments approach; design hybrid; promoter ... See more keywords
Removal efficiency optimization of organic pollutant (methylene blue) with modified multi-walled carbon nanotubes using design of experiments (DOE)
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Materials Science: Materials in Electronics"
DOI: 10.1007/s10854-017-6745-5
Abstract: Photo degradation of the organic pollutants using photocatalysts is affected by several factors. The main goal of the current study is investigation the effect of UV irradiation time (ranging from 5 to 25 min), pH of… read more here.
Keywords: mwcnts zno; using design; methylene blue; removal efficiency ... See more keywords
Use of statistical design of experiments for surface modification of Kapton films by CF4-O2 microwave plasma treatment
Sign Up to like & getrecommendations! Published in 2017 at "Applied Surface Science"
DOI: 10.1016/j.apsusc.2017.05.140
Abstract: Abstract A statistical design of experiments (DoE) was used to evaluate the effects of CF4 O2 plasma on Kapton films in which the duration of treatment, volume ratio of plasma gases, and microwave power were… read more here.
Keywords: statistical design; plasma; treatment; kapton ... See more keywords
Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse.
Sign Up to like & getrecommendations! Published in 2020 at "Bioresource technology"
DOI: 10.1016/j.biortech.2020.124499
Abstract: To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH:… read more here.
Keywords: design experiments; alkaline pretreatment; sugarcane bagasse; design ... See more keywords
Adaptive design of experiments based on Gaussian mixture regression
Sign Up to like & getrecommendations! Published in 2021 at "Chemometrics and Intelligent Laboratory Systems"
DOI: 10.1016/j.chemolab.2020.104226
Abstract: Abstract In the design of molecules, materials, and processes, adaptive design of experiments (ADoE) is conducted to minimize the number of experiments. Although Bayesian optimization (BO) is an effective tool, BO merely selects a candidate… read more here.
Keywords: number; mixture regression; gaussian mixture; design experiments ... See more keywords
Methodology for efficient parametrisation of electrochemical PEMFC model for virtual observers: Model based optimal design of experiments supported by parameter sensitivity analysis
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Hydrogen Energy"
DOI: 10.1016/j.ijhydene.2020.10.146
Abstract: Abstract Determination of the optimal design of experiments that enables efficient parametrisation of fuel cell (FC) model with a minimum parametrisation data-set is one of the key prerequisites for minimizing costs and effort of the… read more here.
Keywords: design experiments; parametrisation; methodology; model ... See more keywords
An open source pipeline for design of experiments for hyperelastic models of the skin with applications to keloids.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the mechanical behavior of biomedical materials"
DOI: 10.1016/j.jmbbm.2020.103999
Abstract: The aim of this work is to characterize the mechanical parameters governing the in-plane behavior of human skin and, in particular, of a keloid-scar. We consider 2D hyperelastic bi-material model of a keloid and the… read more here.
Keywords: model parameter; experimental measurements; model; design experiments ... See more keywords