RT-qPCR Detection of Low-Copy HIV RNA with Yin-Yang Probes.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0138-9_3
Abstract: Accurate monitoring of low levels of viral load (the number of viral particles per milliliter of plasma) in HIV-infected patients is important in terms of evaluation of the progress of antiretroviral therapy. The general approach… read more here.
Keywords: hiv rna; detection; yin yang; detection low ... See more keywords
Detection of Low-Abundance DNA Modifications Using Signal Amplification-Based Immunocytochemistry.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0876-0_14
Abstract: Immunocytochemistry can be instrumental in assessing the spatial distribution and relative levels of epigenetic modifications. Although conventional immunostaining has been utilized for the detection of 5-methylcytosine (5mC) in animal cells and tissues for several decades,… read more here.
Keywords: dna; immunocytochemistry; detection low; low abundance ... See more keywords
Oligonucleotide Primers with G8AE-Clamp Modifications for RT-qPCR Detection of the Low-Copy dsRNA.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9216-4_18
Abstract: We developed a new technique suitable for improved detection of low-copy dsRNA using modified oligonucleotides as primers in RT-qPCR. Insertion of G8AE-clamp residues into primers significantly improves thermal stability of duplexes with RNA without decrease… read more here.
Keywords: low copy; copy dsrna; detection low;
Detection of Low-Abundant MicroRNAs with Hybridization Microchips
Sign Up to like & getrecommendations! Published in 2019 at "Bulletin of Experimental Biology and Medicine"
DOI: 10.1007/s10517-019-04441-z
Abstract: The effect of low concentrations of miRNA on the ability of GeneChip miRNA 4.0 hybridization chips to evaluate their representation in the sample was studied. It is shown that the evaluation of the expression of… read more here.
Keywords: micrornas hybridization; low abundant; hybridization; abundant micrornas ... See more keywords
Detection of low-abundance point mutations by competitive strand assisted endonuclease IV signal amplification system
Sign Up to like & getrecommendations! Published in 2017 at "Current Medical Science"
DOI: 10.1007/s11596-017-1808-7
Abstract: SummaryGenetic mutations are important molecular biomarkers for cancer diagnosis and surveillance. Therefore, the development of methods for mutation detection characterized with straightforward, highly specific and sensitive to low-level mutations within various sequence contexts is extremely… read more here.
Keywords: detection; mutations competitive; point mutations; detection low ... See more keywords
Detection of low levels of Escherichia coli by electrochemical impedance spectroscopy and singular value decomposition
Sign Up to like & getrecommendations! Published in 2017 at "Chemometrics and Intelligent Laboratory Systems"
DOI: 10.1016/j.chemolab.2016.11.008
Abstract: The first steps are reported in the development of a new ultrasensitive electrochemical biosensor for detection of Escherichia coli (E. coli) in water. Two gold electrodes in a sandwich flow-cell w ... read more here.
Keywords: escherichia coli; levels escherichia; spectroscopy; detection low ... See more keywords
MS analysis of a dilution series of bacteria:phytoplankton to improve detection of low abundance bacterial peptides
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-27650-4
Abstract: Assigning links between microbial activity and biogeochemical cycles in the ocean is a primary objective for ecologists and oceanographers. Bacteria represent a small ecosystem component by mass, but act as the nexus for both nutrient… read more here.
Keywords: detection; dilution series; abundance; detection low ... See more keywords
Performance of seven commercial automated assays for the detection of low levels of anti-Toxoplasma IgG in French immunocompromised patients
Sign Up to like & getrecommendations! Published in 2019 at "Parasite"
DOI: 10.1051/parasite/2019052
Abstract: Background: Immunocompromised patients are at high risk for the development of severe toxoplasmosis from tissue cyst reactivation, the most frequently, or from recently acquired acute infections. Knowledge of serologic status is therefore crucial. Screening for… read more here.
Keywords: immunocompromised patients; performance seven; toxoplasmosis; detection low ... See more keywords
Detection of low-metallicity warm plasma in a galaxy overdensity environment at z ̃ 0.2
Sign Up to like & getrecommendations! Published in 2018 at "Monthly Notices of the Royal Astronomical Society"
DOI: 10.1093/mnras/sty042
Abstract: We present results from the analysis of a multiphase O VI - broad Ly-alpha absorber at z = 0.19236 in the HST/COS spectrum of PG 1121+422. The low and intermediate ionization metal lines in this… read more here.
Keywords: galaxy; plasma; gas; low metallicity ... See more keywords
Abstract 6522: Improved detection of low frequency mutations in ovarian and endometrial cancers by utilizing a highly accurate sequencing platform
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-6522
Abstract: Ovarian and endometrial cancers come within the top-4 for incident cancers as well as deaths in North American women. Cure rates have not improved in 30 years as high-grade subtypes continue to be diagnosed in… read more here.
Keywords: ovarian endometrial; endometrial cancers; accurate sequencing; highly accurate ... See more keywords
Abstract LB254: Accurate detection of low frequency AML-associated mutations in vitro using Duplex Sequencing with enzymatic fragmentation
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-lb254
Abstract: Sensitive and specific detection of low frequency mutations in acute myeloid leukemia (AML) is critical in research of minimal residual disease (MRD). At variant allele frequencies (VAF) below approximately 1%, PCR and sequencing errors result… read more here.
Keywords: dna; fragmentation; low frequency; detection low ... See more keywords