Unique Electrical Signature of Phosphate for Specific Single-Molecule Detection of Peptide Phosphorylation.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c05787
Abstract: Single-molecule measurements of biomaterials bring novel insights into cellular events. For almost all of these events, post-translational modifications (PTMs), which alter the properties of proteins through their chemical modifications, constitute essential regulatory mechanisms. However, suitable… read more here.
Keywords: single molecule; detection peptide; methodology; molecule ... See more keywords
Luminescence detection of peptide:N-glycanase activity using engineered split inteins.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical communications"
DOI: 10.1039/d2cc04865e
Abstract: A split intein-based method has been developed to detect peptide:N-glycanase (PNGase) activity in live cells. PNGase cleaves the linkage between N,N'-diacetylchitobiose and the Asn side-chain of N-intein peptides and the products react rapidly with C-intein… read more here.
Keywords: luminescence detection; split; detection peptide; activity ... See more keywords