Determining protein similarity by comparing hydrophobic core structure
Sign Up to like & getrecommendations! Published in 2017 at "Heliyon"
DOI: 10.1016/j.heliyon.2017.e00235
Abstract: Formal assessment of structural similarity is − next to protein structure prediction − arguably the most important unsolved problem in proteomics. In this paper we propose a similarity criterion based on commonalities between the proteins’… read more here.
Keywords: hydrophobic core; similarity; determining protein; protein similarity ... See more keywords
ACTB and GAPDH appear at multiple SDS-PAGE positions, thus not suitable as reference genes for determining protein loading in techniques like Western blotting
Sign Up to like & getrecommendations! Published in 2021 at "Open Life Sciences"
DOI: 10.1515/biol-2021-0130
Abstract: Abstract We performed polyacrylamide gel electrophoresis of human proteins with sodium dodecyl sulfate, isolated proteins at multiple positions, and then used liquid chromatography and tandem mass spectrometry (LC-MS/MS) to determine the protein identities. Although beta-actin… read more here.
Keywords: reference genes; determining protein; protein; genes determining ... See more keywords