Epitope Screening Using Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS): An Accelerated Workflow for Evaluation of Lead Monoclonal Antibodies.
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology journal"
DOI: 10.1002/biot.202100358
Abstract: BACKGROUND Epitope mapping is an increasingly important aspect of biotherapeutic and vaccine development. Recent advances in therapeutic antibody design and production have enabled candidate mAbs to be identified at a rapidly increasing rate, resulting in… read more here.
Keywords: mass spectrometry; exchange mass; epitope screening; hydrogen deuterium ... See more keywords
Mapping the Interactions of Selective Biochemical Probes of Antibody Conformation by Hydrogen–Deuterium Exchange Mass Spectrometry
Sign Up to like & getrecommendations! Published in 2017 at "ChemBioChem"
DOI: 10.1002/cbic.201600670
Abstract: Protein‐based pharmaceuticals represent the fastest growing group of drugs in development in the pharmaceutical industry. One of the major challenges in the discovery, development, and distribution of biopharmaceuticals is the assessment of changes in their… read more here.
Keywords: mass spectrometry; exchange mass; antibody; hydrogen deuterium ... See more keywords
Rapid antibody conformational screening by matrix assisted laser desorption ionization hydrogen-deuterium exchange mass spectrometry.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of separation science"
DOI: 10.1002/jssc.202100986
Abstract: Recent advances in the field of cancer biology have accelerated the discovery and development of novel biopharmaceuticals. At the forefront of these drug development efforts are high-throughput screening, compressed timelines and limited sample quantities, all… read more here.
Keywords: assisted laser; laser desorption; desorption ionization; hydrogen deuterium ... See more keywords
Bridging protein structure, dynamics, and function using hydrogen/deuterium‐exchange mass spectrometry
Sign Up to like & getrecommendations! Published in 2019 at "Protein Science"
DOI: 10.1002/pro.3790
Abstract: Much of our understanding of protein structure and mechanistic function has been derived from static high‐resolution structures. As structural biology has continued to evolve it has become clear that high‐resolution structures alone are unable to… read more here.
Keywords: protein; protein structure; hydrogen deuterium; function ... See more keywords
Studying Protein-DNA Interactions by Hydrogen/Deuterium Exchange Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1126-5_11
Abstract: Protein hydrogen/deuterium exchange (HDX) coupled to mass spectrometry (MS) can be used to study interactions of proteins with various ligands, to describe the effects of mutations, or to reveal structural responses of proteins to different… read more here.
Keywords: protein; mass spectrometry; dna; protein dna ... See more keywords
Epitope mapping of antibodies in C-reactive protein assay kits by hydrogen-deuterium exchange mass spectrometry explains differential results across kits
Sign Up to like & getrecommendations! Published in 2022 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-022-04029-z
Abstract: C-Reactive protein (CRP) is an important marker for in vitro diagnosis (IVD) of inflammation. However, CRP immunoturbidimetric kits from different manufacturers exhibit inconsistency in evaluation, making clinical diagnosis challenging. The use of immunological methods in… read more here.
Keywords: reactive protein; epitope mapping; exchange mass; hydrogen deuterium ... See more keywords
Hydrogen/Deuterium Exchange Behavior During Denaturing/Refolding Processes Determined in Tetragonal Hen Egg-White Lysozyme Crystals
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Biotechnology"
DOI: 10.1007/s12033-022-00447-7
Abstract: The hydrogen/deuterium (H/D) exchange of main-chain amide hydrogens in the protein that denatured and refolded in deuterated solvent is considered to contain the traces of hydrogen bond cleavages or the exposure to solvent of the… read more here.
Keywords: hen egg; denaturing refolding; refolding processes; hydrogen deuterium ... See more keywords
Comparing Hydrogen Deuterium Exchange and Fast Photochemical Oxidation of Proteins: a Structural Characterisation of Wild-Type and ΔN6 β2-Microglobulin
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1007/s13361-018-2067-y
Abstract: AbstractHydrogen deuterium exchange (HDX) coupled to mass spectrometry (MS) is a well-established technique employed in the field of structural MS to probe the solvent accessibility, dynamics and hydrogen bonding of backbone amides in proteins. By… read more here.
Keywords: oxidation proteins; deuterium exchange; fast photochemical; photochemical oxidation ... See more keywords
Dynamics and Conformational Changes in Human NEIL2 DNA Glycosylase Analyzed by Hydrogen/Deuterium Exchange Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2021.167334
Abstract: Base excision DNA repair (BER) is necessary for removal of damaged nucleobases from the genome and their replacement with normal nucleobases. BER is initiated by DNA glycosylases, the enzymes that cleave the N-glycosidic bonds of… read more here.
Keywords: hneil2; dna; exchange mass; hydrogen deuterium ... See more keywords
Protein dynamics and conformational changes explored by hydrogen/deuterium exchange mass spectrometry.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in structural biology"
DOI: 10.1016/j.sbi.2019.06.007
Abstract: Proteins are not rigid bodies under their physiological conditions. Here we discuss a solution-phase structural proteomics technique, hydrogen deuterium exchange coupled with mass spectrometry (HDX-MS), as a means to study protein dynamics, which can complement… read more here.
Keywords: protein dynamics; deuterium exchange; hydrogen deuterium; mass spectrometry ... See more keywords
Complementarity of Hydrogen/Deuterium Exchange Mass Spectrometry and Cryo-Electron Microscopy.
Sign Up to like & getrecommendations! Published in 2020 at "Trends in biochemical sciences"
DOI: 10.1016/j.tibs.2020.05.005
Abstract: Methodological improvements in both single particle cryo-electron microscopy (cryo-EM) and hydrogen/deuterium exchange mass spectrometry (HDX-MS) mean that the two methods are being more frequently used together to tackle complex problems in structural biology. There are… read more here.
Keywords: cryo electron; microscopy; hydrogen deuterium; deuterium exchange ... See more keywords