Minimal purification method enables developability assessment of recombinant proteins.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28385
Abstract: Analytical characterization of proteins is a critical task for developing therapeutics and subunit vaccine candidates. Assessing candidates with a battery of biophysical assays can inform the selection of one that exhibits properties consistent with a… read more here.
Keywords: purification; recombinant proteins; purification method; minimal purification ... See more keywords
Improving the Developability of an Antigen Binding Fragment by Aspartate Substitutions.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemistry"
DOI: 10.1021/acs.biochem.9b00251
Abstract: Aggregation can be a major challenge in the development of antibody-based pharmaceuticals as it can compromise the quality of the product during bioprocessing, formulation, and drug administration. To avoid aggregation, developability assessment is often run… read more here.
Keywords: developability; aggregation; antigen binding; asp substitutions ... See more keywords
Current advances in biopharmaceutical informatics: guidelines, impact and challenges in the computational developability assessment of antibody therapeutics
Sign Up to like & getrecommendations! Published in 2022 at "mAbs"
DOI: 10.1080/19420862.2021.2020082
Abstract: ABSTRACT Therapeutic monoclonal antibodies and their derivatives are key components of clinical pipelines in the global biopharmaceutical industry. The availability of large datasets of antibody sequences, structures, and biophysical properties is increasingly enabling the development… read more here.
Keywords: assessment antibody; biopharmaceutical informatics; developability assessment; antibody ... See more keywords
Assessing developability early in the discovery process for novel biologics
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DOI: 10.1080/19420862.2023.2171248
Abstract: ABSTRACT Beyond potency, a good developability profile is a key attribute of a biological drug. Selecting and screening for such attributes early in the drug development process can save resources and avoid costly late-stage failures.… read more here.
Keywords: discovery process; developability early; early discovery; assessing developability ... See more keywords
Blueprint for antibody biologics developability
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DOI: 10.1080/19420862.2023.2185924
Abstract: ABSTRACT Large-molecule antibody biologics have revolutionized medicine owing to their superior target specificity, pharmacokinetic and pharmacodynamic properties, safety and toxicity profiles, and amenability to versatile engineering. In this review, we focus on preclinical antibody developability,… read more here.
Keywords: antibody biologics; biologics developability; blueprint antibody; antibody ... See more keywords
Identifying developability risks for clinical progression of antibodies using high-throughput in vitro and in silico approaches.
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DOI: 10.1080/19420862.2023.2200540
Abstract: With the growing significance of antibodies as a therapeutic class, identifying developability risks early during development is of paramount importance. Several high-throughput in vitro assays and in silico approaches have been proposed to de-risk antibodies… read more here.
Keywords: throughput vitro; developability risks; high throughput; identifying developability ... See more keywords
Complementary epitopes and favorable developability of monoclonal anti-LAMP1 antibodies generated using two transgenic animal platforms
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DOI: 10.1371/journal.pone.0235815
Abstract: Monoclonal antibodies (mAbs) for therapeutic applications should be as similar to native human antibodies as possible to minimize their immunogenicity in patients. Several transgenic animal platforms are available for the generation of fully human mAbs.… read more here.
Keywords: developability; transgenic animal; animal platforms; platforms complementary ... See more keywords