Recent advances of data‐independent acquisition mass spectrometry‐based proteomics
Sign Up to like & getrecommendations! Published in 2023 at "PROTEOMICS"
DOI: 10.1002/pmic.202200011
Abstract: Bottom-up proteomics is a mass spectrometry-based method to analyze the contents of complex protein samples. Pioneered in the 1990s, it consists of converting protein samples into peptide samples by enzymatic digestion, the separation of peptides… read more here.
Keywords: dia; number; peptide precursors; approach ... See more keywords
Integration of data-independent acquisition (DIA) with co-fractionation mass spectrometry (CF-MS) to enhance interactome mapping capabilities.
Sign Up to like & getrecommendations! Published in 2023 at "Proteomics"
DOI: 10.1002/pmic.202200278
Abstract: Proteomics technologies are continually advancing, providing opportunities to develop stronger and more robust protein interaction networks (PINs). In part, this is due to the ever-growing number of high-throughput proteomics methods that are available. This review… read more here.
Keywords: data independent; dia; dia fractionation; acquisition dia ... See more keywords
Improving Precursor Selectivity in Data-Independent Acquisition Using Overlapping Windows
Sign Up to like & getrecommendations! Published in 2019 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1007/s13361-018-2122-8
Abstract: AbstractA major goal of proteomics research is the accurate and sensitive identification and quantification of a broad range of proteins within a sample. Data-independent acquisition (DIA) approaches that acquire MS/MS spectra independently of precursor information… read more here.
Keywords: dia; data independent; precursor selectivity; independent acquisition ... See more keywords
Optimised data-independent acquisition strategy recaptures the classification of early-stage hepatocellular carcinoma based on data-dependent acquisition.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of proteomics"
DOI: 10.1016/j.jprot.2021.104152
Abstract: Proteomics is increasingly used for exploring disease biomarkers and therapeutic targets. The data-independent acquisition (DIA) method collects all peptide signals in a sample, and provides a convenient way to archive disease-related molecular features for further… read more here.
Keywords: dia; hepatocellular carcinoma; data independent; independent acquisition ... See more keywords
The Isotopic Ac-IP Tag Enables Multiplexed Proteome Quantification in Data-Independent Acquisition Mode
Sign Up to like & getrecommendations! Published in 2021 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.1c00453
Abstract: Data-independent acquisition (DIA) is an increasingly used approach for quantitative proteomics. However, most current isotope labeling strategies are not suitable for DIA as they lead to more complex MS2 spectra or severe ratio distortion. As… read more here.
Keywords: dia; data independent; independent acquisition; tag ... See more keywords
DIA+: A Data-Independent Acquisition Method Combining Multiple Precursor Charges to Improve Peptide Signal.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b03418
Abstract: Data-independent acquisition methods that acquire fragment ions from virtually any peptide in a sample have expanded the benefits of low-throughput targeted proteomics to proteome-wide analyses. While these methods have increased the reproducibility of peptide quantification… read more here.
Keywords: dia; dia data; data independent; method ... See more keywords
MSLibrarian: Optimized Predicted Spectral Libraries for Data-Independent Acquisition Proteomics
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Proteome Research"
DOI: 10.1021/acs.jproteome.1c00796
Abstract: Data-independent acquisition-mass spectrometry (DIA-MS) is the method of choice for deep, consistent, and accurate single-shot profiling in bottom-up proteomics. While classic workflows for targeted quantification from DIA-MS data require auxiliary data-dependent acquisition (DDA) MS analysis… read more here.
Keywords: spectral libraries; data independent; optimized predicted; dia ... See more keywords
Definitive Screening Designs to Optimize Library-Free DIA-MS Identification and Quantification of Neuropeptides.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.3c00088
Abstract: Method optimization is crucial for successful mass spectrometry (MS) analysis. However, extensive method assessments, altering various parameters individually, are rarely performed due to practical limitations regarding time and sample quantity. To maximize sample space for… read more here.
Keywords: dia; identification; screening designs; definitive screening ... See more keywords
A novel strategy for the characterization of glaucocalyxin A metabolites in vivo and in vitro by UHPLC-Q-TOF-MS based on DDA and DIA data acquisitions
Sign Up to like & getrecommendations! Published in 2020 at "RSC Advances"
DOI: 10.1039/c9ra06830a
Abstract: Glaucocalyxin A (GLA) belongs to the natural ent-kauranoid diterpenoids family with antitumor, antifibrotic, anticoagulative, antioxidant, and anti-AD effects. In this study, ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) system was applied to… read more here.
Keywords: dia; uhplc tof; glaucocalyxin; phase metabolites ... See more keywords
Alpha-Tri: a deep neural network for scoring the similarity between predicted and measured spectra improves peptide identification of DIA data
Sign Up to like & getrecommendations! Published in 2022 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btab878
Abstract: MOTIVATION Peptide identification of data-independent acquisition (DIA) mass spectrometry (MS) applying the peptide-centric approach heavily relies on the spectral library matching, such as the fragment intensity similarity. If the intensity similarity is calculated through all… read more here.
Keywords: intensity similarity; similarity; fragment ions; dia ... See more keywords
Economic burden of drug-induced anaphylaxis: what can we do better?
Sign Up to like & getrecommendations! Published in 2022 at "Current Opinion in Allergy and Clinical Immunology"
DOI: 10.1097/aci.0000000000000836
Abstract: Purpose of review This systematic review evaluates published data related to the economic burden of drug-induced anaphylaxis (DIA) to understand preventive cost saving measures that could reduce the burden of these conditions. Recent findings Although… read more here.
Keywords: drug induced; induced anaphylaxis; dia; economic burden ... See more keywords