A Fluorescence-Based Assay for Quantitative Analysis of Phospholipid:Diacylglycerol Acyltransferase Activity.
Sign Up to like & getrecommendations! Published in 2019 at "Lipids"
DOI: 10.1002/lipd.12190
Abstract: Phospholipid:diacylglycerol acyltransferase (PDAT) catalyzes the acyl-CoA-independent triacylglycerol (TAG) biosynthesis in plants and oleaginous microorganisms and thus is a key target in lipid research. The conventional in vitro PDAT activity assay involves the use of radiolabeled… read more here.
Keywords: phospholipid diacylglycerol; fluorescence based; diacylglycerol; diacylglycerol acyltransferase ... See more keywords
Flavonoids from the seeds of Psoralea corylifolia inhibit diacylglycerol acyltransferase
Sign Up to like & getrecommendations! Published in 2021 at "Phytochemistry Letters"
DOI: 10.1016/j.phytol.2021.06.013
Abstract: Abstract Three new flavonoids, including a new prenylated aurone, 7,2′,5′-trihydroxy-8-prenylaurone (1), and two new flavanones, 2(S)-4′-hydroxy-6-methoxy-7-(2”-hydroxy-3”-methybult-3”-enyl)flavanone (2) and 2(S)-4′-hydroxy-7-methoxy-6-(1”,2”-epoxy-3”-hydroxy-dimethyl)flavanone (3), together with eight known flavonoids were isolated from the seeds of Psoralea corylifolia L. The… read more here.
Keywords: inhibit diacylglycerol; psoralea corylifolia; seeds psoralea; corylifolia inhibit ... See more keywords
Direct Analysis from Phase-Separated Liquid Samples using ADE-OPI-MS: Applicability to High-Throughput Screening for Inhibitors of Diacylglycerol Acyltransferase 2.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c04312
Abstract: The primary goal of high-throughput screening (HTS) is to rapidly survey a broad collection of compounds, numbering from tens of thousands to millions of members, and identify those that modulate the activity of a therapeutic… read more here.
Keywords: high throughput; ade opi; diacylglycerol acyltransferase; phase separated ... See more keywords
Homologous Overexpression of Diacylglycerol Acyltransferase in Oleaginous Fungus Mucor circinelloides WJ11 Enhances Lipid Accumulation under Static Solid Cultivation.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.2c03489
Abstract: Diacylglycerol acyltransferase (DGAT) catalyzes the binding of acyl-CoA to diacylglycerol to form triacylglycerol (TAG). Previous studies strongly indicate that DGAT2, rather than DGAT1, is crucial for TAG accumulation in the oleaginous fungus Mucor circinelloides. To… read more here.
Keywords: oleaginous fungus; accumulation; diacylglycerol acyltransferase; fermentation ... See more keywords
Intrinsic disorder in the regulatory N-terminal domain of diacylglycerol acyltransferase 1 from Brassica napus
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-34339-1
Abstract: Proteins with multifunctional regulatory domains often demonstrate structural plasticity or protein disorder, allowing the binding of multiple regulatory factors and post-translational modifications. While the importance of protein disorder is clear, it also poses a challenge… read more here.
Keywords: terminal domain; intrinsic disorder; diacylglycerol acyltransferase; brassica napus ... See more keywords
Chemical constituents from the seeds of Cullen corylifolium and their inhibitory activity on diacylglycerol acyltransferase
Sign Up to like & getrecommendations! Published in 2022 at "Natural Product Research"
DOI: 10.1080/14786419.2022.2103126
Abstract: Abstract A large number of extracts of medicinal plants or natural products shows beneficial to combat obesity. In the present work, a new flavonoid named (2S,1″R,2″R)-4′-hydroxy-7-methoxy-6-(1,2,3-trihydroxy-3-methyl-butyl)-flavanone (1), along with seven known compounds (2–8) were isolated… read more here.
Keywords: inhibitory activity; diacylglycerol acyltransferase; seeds cullen; cullen corylifolium ... See more keywords
Lipid production is more than doubled by manipulating a diacylglycerol acyltransferase in algae
Sign Up to like & getrecommendations! Published in 2020 at "GCB Bioenergy"
DOI: 10.1111/gcbb.12771
Abstract: Oleaginous algae have the ability to synthesize a high level of triacylglycerol (TAG) and are considered as the next‐generation feedstock for biofuel production. Manipulating algal lipid biosynthetic pathways has potential to overproduce TAG and represents… read more here.
Keywords: diacylglycerol acyltransferase; lipid production; production; algae ... See more keywords
DS-7250, a Diacylglycerol Acyltransferase 1 Inhibitor, Enhances Hepatic Steatosis in Zucker Fatty Rats via Upregulation of Fatty Acid Synthesis
Sign Up to like & getrecommendations! Published in 2018 at "Toxicologic Pathology"
DOI: 10.1177/0192623318765909
Abstract: Diacylglycerol acyltransferase 1 (DGAT1) catalyzes the final step in triglyceride synthesis. Since Dgat1 -/- mice fed a high-fat diet (HFD) are resistant to hepatic steatosis, DGAT1 inhibitors are expected to have antifatty liver effects. To… read more here.
Keywords: hepatic steatosis; fatty rats; diacylglycerol acyltransferase; zucker fatty ... See more keywords
Distinct Physiological Roles of Three Phospholipid:Diacylglycerol Acyltransferase Genes in Olive Fruit with Respect to Oil Accumulation and the Response to Abiotic Stress
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2021.751959
Abstract: Three different cDNA sequences, designated OepPDAT1-1, OepPDAT1-2, and OepPDAT2, encoding three phospholipid:diacylglycerol acyltransferases (PDAT) have been isolated from olive (Olea europaea cv. Picual). Sequence analysis showed the distinctive features typical of the PDAT family and… read more here.
Keywords: fruit; pdat; diacylglycerol; diacylglycerol acyltransferase ... See more keywords
Applications of Diacylglycerol Acyltransferase for Triacylglycerol Production in Mortierella alpina
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Fungi"
DOI: 10.3390/jof9020219
Abstract: Triacylglycerol (TG) with high-value long-chain polyunsaturated fatty acids is beneficial to human health; consequently, there is an urgent need to broaden its sources due to the current growing demand. Mortierella alpina, one of the most… read more here.
Keywords: mortierella alpina; applications diacylglycerol; production; triacylglycerol ... See more keywords
Genome-wide identification and expression analysis of diacylglycerol acyltransferase genes in soybean (Glycine max)
Sign Up to like & getrecommendations! Published in 2023 at "PeerJ"
DOI: 10.7717/peerj.14941
Abstract: Background Soybean (Glycine max) is a major protein and vegetable oil source. In plants, diacylglycerol acyltransferase (DGAT) can exert strong flux control, which is rate-limiting for triacylglycerol biosynthesis in seed oil formation. Methods Here, we… read more here.
Keywords: dgat; expression; diacylglycerol acyltransferase; glycine max ... See more keywords