Caffeine inhibits PI3K and mTORC2 in Dictyostelium and differentially affects multiple other cAMP chemoattractant signaling effectors
Sign Up to like & getrecommendations! Published in 2019 at "Molecular and Cellular Biochemistry"
DOI: 10.1007/s11010-019-03520-z
Abstract: Caffeine is commonly used in Dictyostelium to inhibit the synthesis of the chemoattractant cAMP and, therefore, its secretion and the autocrine stimulation of cells, in order to prevent its interference with the study of chemoattractant-induced… read more here.
Keywords: pi3k mtorc2; camp; dictyostelium; caffeine inhibits ... See more keywords
Loss of Cln3 impacts protein secretion in the social amoeba Dictyostelium.
Sign Up to like & getrecommendations! Published in 2017 at "Cellular signalling"
DOI: 10.1016/j.cellsig.2017.03.022
Abstract: Neuronal ceroid lipofuscinosis (NCL), also referred to as Batten disease, is the most common form of childhood neurodegeneration. Mutations in CLN3 cause the most prevalent subtype of the disease, which manifests during early childhood and… read more here.
Keywords: secretion; loss cln3; protein secretion; cln3 ... See more keywords
The 14-3-3 protein is an essential component of cyclic AMP signaling for regulation of chemotaxis and development in Dictyostelium.
Sign Up to like & getrecommendations! Published in 2020 at "Cellular signalling"
DOI: 10.1016/j.cellsig.2020.109739
Abstract: The evolutionarily-conserved 14-3-3 proteins regulate many cellular processes through binding to various phosphorylated targets in eukaryotes. It first appears in Dictyostelium, however its role in this organism is poorly understood. Here we show that down-regulation… read more here.
Keywords: dictyostelium; cyclic amp; component cyclic; regulation ... See more keywords
Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis
Sign Up to like & getrecommendations! Published in 2022 at "Nucleus"
DOI: 10.1080/19491034.2022.2047289
Abstract: ABSTRACT Dictyostelium amoebae perform a semi-closed mitosis, in which the nuclear envelope is fenestrated at the insertion sites of the mitotic centrosomes and around the central spindle during karyokinesis. During late telophase the centrosome relocates… read more here.
Keywords: dictyostelium spastin; closed mitosis; nuclear envelope; semi closed ... See more keywords
Mapping and characterization of G-quadruplexes in the genome of the social amoeba Dictyostelium discoideum
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkz196
Abstract: Abstract G-quadruplexes (G4) are non-canonical DNA and/or RNA secondary structures formed in guanine-rich regions. Given their over-representation in specific regions in the genome such as promoters and telomeres, they are likely to play important roles… read more here.
Keywords: genome; dictyostelium; dictyostelium discoideum; social amoeba ... See more keywords
Generating polyketide diversity in Dictyostelium: a Steely hybrid polyketide synthase produces alternate products at different developmental stages
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the Royal Society B: Biological Sciences"
DOI: 10.1098/rspb.2022.1176
Abstract: The soil is a rich ecosystem where many ecological interactions are mediated by small molecules, and in which amoebae are low-level predators and also prey. The social amoeba Dictyostelium discoideum has a high genomic potential… read more here.
Keywords: synthase; generating polyketide; dictyostelium steely; diversity dictyostelium ... See more keywords
Mitogen-activated protein kinase regulation of the phosphodiesterase RegA in early Dictyostelium development.
Sign Up to like & getrecommendations! Published in 2019 at "Microbiology"
DOI: 10.1099/mic.0.000868
Abstract: Mitogen-activated protein kinase (MAPK) regulation of cAMP-specific phosphodiesterase function has been demonstrated in mammalian cells and suspected to occur in other eukaryotes. Epistasis analysis in the soil amoeba Dictyostelium discoideum suggests the atypical MAPK Erk2… read more here.
Keywords: docking motif; phosphodiesterase; function; dictyostelium ... See more keywords
Dictyostelium lacking the single atlastin homolog Sey1 shows aberrant ER architecture, proteolytic processes and expansion of the Legionella‐containing vacuole
Sign Up to like & getrecommendations! Published in 2021 at "Cellular Microbiology"
DOI: 10.1111/cmi.13318
Abstract: Dictyostelium discoideum Sey1 is the single ortholog of mammalian atlastin 1–3 (ATL1‐3), which are large homodimeric GTPases mediating homotypic fusion of endoplasmic reticulum (ER) tubules. In this study, we generated a D. discoideum mutant strain… read more here.
Keywords: containing vacuole; legionella containing; sey1; dictyostelium ... See more keywords
Homeodomain-like DNA binding proteins control the haploid-to-diploid transition in Dictyostelium
Sign Up to like & getrecommendations! Published in 2017 at "Science Advances"
DOI: 10.1126/sciadv.1602937
Abstract: Structures of social amoeba mating-type proteins suggest deep conservation of sexual differentiation processes. Homeodomain proteins control the developmental transition between the haploid and diploid phases in several eukaryotic lineages, but it is not known whether… read more here.
Keywords: transition; dna; haploid diploid; mata matb ... See more keywords
Spatial heterogeneities shape the collective behavior of signaling amoeboid cells
Sign Up to like & getrecommendations! Published in 2020 at "Science Signaling"
DOI: 10.1126/scisignal.aaz3975
Abstract: Imaging and mathematical analyses reveal how Dictyostelium discoideum cells navigate around obstacles in their environment. Slime mold obstacle course The slime mold Dictyostelium discoideum, which lives in the soil, is an experimental model of cell… read more here.
Keywords: cells spatial; slime mold; dictyostelium discoideum; spatial heterogeneities ... See more keywords
THP-1 and Dictyostelium Infection Models for Screening and Characterization of Anti-Mycobacterium abscessus Hit Compounds
Sign Up to like & getrecommendations! Published in 2019 at "Antimicrobial Agents and Chemotherapy"
DOI: 10.1128/aac.01601-19
Abstract: !!NCR1!! presents a great challenge to antimycobacterial therapy due to its innate resistance against most antibiotics. M. abscessus is able to grow intracellularly in human macrophages, suggesting that intracellular models can facilitate drug discovery. Thus,… read more here.
Keywords: dictyostelium; infection; human macrophages; abscessus ... See more keywords