Exploring three different expression systems for recombinant expression of globins: Escherichia coli, Pichia pastoris and Spodoptera frugiperda.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2017.11.027
Abstract: Globins are among the best investigated proteins in biological and medical sciences and represent a prime tool for the study of the evolution of genes and the structure-function relationship of proteins. Here, we explore the… read more here.
Keywords: different expression; expression globins; expression; expression systems ... See more keywords
CRISPR/Cas9-mediated knock-in strategy at the Rosa26 locus in cattle fetal fibroblasts
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DOI: 10.1371/journal.pone.0276811
Abstract: The genetic modification of cattle has many agricultural and biomedical applications. However, random integration often leads to the unstable or differentially expression of the exogenous genes, which limit the application and development of transgenic technologies.… read more here.
Keywords: crispr cas9; expression units; knock; different expression ... See more keywords