PIE-FLIM Measurements of Two Different FRET-Based Biosensor Activities in the Same Living Cells.
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DOI: 10.1016/j.bpj.2020.03.003
Abstract: We report the use of pulsed interleaved excitation (PIE)-fluorescence lifetime imaging microscopy (FLIM) to measure the activities of two different biosensor probes simultaneously in single living cells. Many genetically encoded biosensors rely on the measurement… read more here.
Keywords: pie flim; fret based; living cells; different fret ... See more keywords