miR‐143 targets MAPK7 in CHO cells and induces a hyperproductive phenotype to enhance production of difficult‐to‐express proteins
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology Progress"
DOI: 10.1002/btpr.2475
Abstract: In recent years, the number of complex but clinically effective biologicals such as multi‐specific antibody formats and fusion proteins has increased dramatically. However, compared to classical monoclonal antibodies (mAbs), these rather artificially designed therapeutic proteins… read more here.
Keywords: mir 143; production; production difficult; cho cells ... See more keywords
A robust antibody discovery platform for difficult‐to‐express G protein‐coupled receptors
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DOI: 10.1002/pro.4520
Abstract: G protein‐coupled receptors (GPCRs) are in the spotlight as drug targets due to the fact that multiple research results have verified the correlation between the activation of GPCRs and disease indications. This is because the… read more here.
Keywords: difficult express; coupled receptors; gpcrs; protein coupled ... See more keywords
Strategies and Considerations for Improving Expression of "Difficult to Express" Proteins in CHO Cells.
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DOI: 10.1007/978-1-4939-6972-2_1
Abstract: Despite substantial advances in the field of mammalian expression, there are still proteins that are characterized as difficult to express. Determining the expression bottleneck requires troubleshooting techniques specific for the given molecule and host. The… read more here.
Keywords: improving expression; considerations improving; expression difficult; expression ... See more keywords
Cell-free protein synthesis for producing ‘difficult-to-express’ proteins
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DOI: 10.1016/j.bej.2018.07.013
Abstract: Abstract Biomanufacturing requires the stable production of active biological molecules including proteins. However, a significant percentage of genes cannot be expressed efficiently in a cell-based system due to cellular homeostasis. Upon overproduction, proteins often form… read more here.
Keywords: production; cell free; express proteins; free protein ... See more keywords
Harnessing secretory pathway differences between HEK293 and CHO to rescue production of difficult to express proteins.
Sign Up to like & getrecommendations! Published in 2022 at "Metabolic engineering"
DOI: 10.1016/j.ymben.2022.03.009
Abstract: Biologics represent the fastest growing group of therapeutics, but many advanced recombinant protein moieties remain difficult to produce. Here, we identify metabolic engineering targets limiting expression of recombinant human proteins through a systems biology analysis… read more here.
Keywords: expression; difficult express; secretory pathway; express proteins ... See more keywords