Digenome-seq web tool for profiling CRISPR specificity
Sign Up to like & getrecommendations! Published in 2017 at "Nature Methods"
DOI: 10.1038/nmeth.4262
Abstract: To the Editor: We recently reported Digenome-seq (digested genome sequencing), a method for in vitro identification of potential off-target sites, and we evaluated the specificity of CRISPR– Cas9 (refs. 1,2) and CRISPR–Cpf1(ref. 3) endonuclease by… read more here.
Keywords: web tool; digenome seq; tool; specificity ... See more keywords
Identifying genome-wide off-target sites of CRISPR RNA–guided nucleases and deaminases with Digenome-seq
Sign Up to like & getrecommendations! Published in 2021 at "Nature Protocols"
DOI: 10.1038/s41596-020-00453-6
Abstract: Digested genome sequencing (Digenome-seq) is a highly sensitive, easy-to-carry-out, cell-free method for experimentally identifying genome-wide off-target sites of programmable nucleases and deaminases (also known as base editors). Genomic DNA is digested in vitro using clustered… read more here.
Keywords: genome wide; dna; identifying genome; target sites ... See more keywords