Simultaneous N-Deglycosylation and Digestion of Complex Samples on S-Traps Enables Efficient Glycosite Hypothesis Generation
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DOI: 10.1021/acsomega.2c08071
Abstract: N-linked glycosylation is an important post-translational modification that is difficult to identify and quantify in traditional bottom-up proteomics experiments. Enzymatic deglycosylation of proteins by peptide:N-glycosidase F (PNGase F) prior to digestion and subsequent mass spectrometry… read more here.
Keywords: deglycosylation digestion; sample preparation; digestion complex; digestion ... See more keywords