Quantification of the allergen soy (Glycine max) in food using digital droplet PCR (ddPCR)
Sign Up to like & getrecommendations! Published in 2018 at "European Food Research and Technology"
DOI: 10.1007/s00217-018-3182-5
Abstract: To meet the increasing need for quantification of allergens and to have an alternative to commercially available ELISA and PCR systems, the Austrian Agency for Health and Food Safety started establishing in-house PCR systems. To… read more here.
Keywords: reference material; pcr; soy; quantification ... See more keywords
One-Step Digital Droplet Auto-Catalytic Nucleic Acid Amplification with High-Throughput Fluorescence Imaging and Droplet Tracking Computation.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01754
Abstract: Digital droplet technology has emerged as a powerful new tool for biomarker analysis. Temperature cycling, enzymes, and off-chip processes are, nevertheless, always required. Herein, we constructed a digital droplet auto-catalytic hairpin assembly (ddaCHA) microfluidic system… read more here.
Keywords: auto catalytic; droplet; digital droplet; droplet auto ... See more keywords
Multiplex Enrichment and Detection of Rare KRAS Mutations in Liquid Biopsy Samples using Digital Droplet Pre-Amplification.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b01605
Abstract: Oncology research is increasingly incorporating molecular detection of circulating tumor DNA (ctDNA) as a tool for cancer surveillance and early detection. However, noninvasive monitoring of conditions with low tumor burden remains challenging, as the diagnostic… read more here.
Keywords: detection; droplet pre; droplet; pre amplification ... See more keywords
On-demand Milifluidic Synthesis of Quantum Dots in Digital Droplet Reactors.
Sign Up to like & getrecommendations! Published in 2020 at "Industrial & engineering chemistry research"
DOI: 10.1021/acs.iecr.9b04230
Abstract: Colloidal quantum dots (QDs) offer dramatic potential due to their size-dependent optical properties. Lack of facile synthesis methods for precise and reproducible size and composition, however, present an extant barrier to their widespread use. Here… read more here.
Keywords: demand milifluidic; droplet; quantum dots; synthesis ... See more keywords
Digital droplet polymerase chain reaction to monitor ultraviolet C treatment of single-donor and buffy coat platelet units.
Sign Up to like & getrecommendations! Published in 2020 at "Transfusion"
DOI: 10.1111/trf.15904
Abstract: BACKGROUND UVC illumination of agitated platelet concentrates (PCs) inactivates pathogens and white blood cells by modifications of their nucleic acids. Related effects on mitochondrial DNA (mtDNA) in platelets serve as a basis for an efficient… read more here.
Keywords: treatment; pcr; buffy coat; platelet ... See more keywords
Avian metapneumovirus: A five-plex digital droplet RT-PCR method for identification of subgroups A, B, C, and D
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Veterinary Science"
DOI: 10.3389/fvets.2022.1058294
Abstract: End-point and real-time avian metapneumovirus (AMPV) RT-PCRs have been developed to detect one or two of the four recognized subgroups (A,B,C, and D) simultaneously or for broad range AMPV detection. Current subgroup specific tests target… read more here.
Keywords: avian metapneumovirus; five plex; digital droplet; droplet pcr ... See more keywords
Development of a Sensitive Digital Droplet PCR Screening Assay for the Detection of GPR126 Non-Coding Mutations in Bladder Cancer Urine Liquid Biopsies
Sign Up to like & getrecommendations! Published in 2023 at "Biomedicines"
DOI: 10.3390/biomedicines11020495
Abstract: Recent whole-genome sequencing studies identified two novel recurrent mutations in the enhancer region of GPR126 in urothelial bladder cancer (UBC) tumor samples. This mutational hotspot is the second most common after the TERT promoter in… read more here.
Keywords: pcr screening; droplet pcr; digital droplet; gpr126 ... See more keywords
Digital Droplet PCR in Hematologic Malignancies: A New Useful Molecular Tool
Sign Up to like & getrecommendations! Published in 2022 at "Diagnostics"
DOI: 10.3390/diagnostics12061305
Abstract: Digital droplet PCR (ddPCR) is a recent version of quantitative PCR (QT-PCR), useful for measuring gene expression, doing clonality assays and detecting hot spot mutations. In respect of QT-PCR, ddPCR is more sensitive, does not… read more here.
Keywords: droplet pcr; ddpcr; digital droplet; pcr hematologic ... See more keywords