Monitoring the threatened Harttiella (Siluriformes, Loricariidae) with environmental DNA: A comparison between metabarcoding and targeted digital PCR
Sign Up to like & getrecommendations! Published in 2024 at "Aquatic Conservation: Marine and Freshwater Ecosystems"
DOI: 10.1002/aqc.4111
Abstract: Environmental DNA has emerged as an efficient alternative to traditional sampling methods. A standard multispecies or a targeted single‐species approach can be used for analysing environmental DNA samples. The costs, benefits, and drawbacks associated with… read more here.
Keywords: metabarcoding targeted; digital pcr; threatened harttiella; environmental dna ... See more keywords
Comparative Analysis of Two Digital PCR Platforms for Detecting DNA Methylation in Patient Samples
Sign Up to like & getrecommendations! Published in 2025 at "Cell Biochemistry and Function"
DOI: 10.1002/cbf.70112
Abstract: The choice of appropriate analytical methods for determining methylation patterns at specific loci across the genome is essential for identifying novel diagnostic and prognostic markers for subsequent clinical implementation. Various methods exist for determining methylation… read more here.
Keywords: methylation; dna methylation; analysis; pcr ... See more keywords
Low-copy transgene detection using nested digital PCR for gene doping control.
Sign Up to like & getrecommendations! Published in 2021 at "Drug testing and analysis"
DOI: 10.1002/dta.3173
Abstract: Gene doping is prohibited for fair competition in human and horse sports. One style of gene doping is the administration of an exogeneous gene, called a transgene, to postnatal humans and horses. Although many transgene… read more here.
Keywords: detection; pcr; gene doping; digital pcr ... See more keywords
Droplet Digital PCR Provides Highly Sensitive and Accurate Opsin Gene SNP Detection From Wild Primate Fecal Samples
Sign Up to like & getrecommendations! Published in 2025 at "Ecology and Evolution"
DOI: 10.1002/ece3.70996
Abstract: ABSTRACT Evaluating field‐sourced samples with poor‐quality and low‐quantity DNA, like animal feces, presents significant challenges in the field of molecular biology. Nonetheless, recent innovations in PCR technology are promoted as effective tools to overcome many… read more here.
Keywords: droplet digital; ddpcr; pcr; digital pcr ... See more keywords
A poly(dimethylsiloxane) microfluidic sheet reversibly adhered on a glass plate for creation of emulsion droplets for droplet digital PCR
Sign Up to like & getrecommendations! Published in 2017 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201600309
Abstract: A PDMS microfluidic chip with T‐junction channel geometry, two inlet reservoirs, and one outlet reservoir was reversibly adhered on a glass plate through the viscoelastic properties of PDMS. This formed a detachable microfluidic device for… read more here.
Keywords: glass plate; creation; droplet digital; glass ... See more keywords
Design of a digital‐PCR assay to quantify fragmented human mitochondrial DNA
Sign Up to like & getrecommendations! Published in 2021 at "Environmental and Molecular Mutagenesis"
DOI: 10.1002/em.22449
Abstract: Digital PCR (dPCR) has been adapted to quantify the proportion of mitochondrial DNA (mtDNA) molecules without and with double‐strand DNA breaks (DSBs). This is based on a break‐apart approach of two differentially labeled target sequences… read more here.
Keywords: molecule; mtdna; dna; digital pcr ... See more keywords
Quantitative detections of TP53 gene mutations improve the diagnosis and prognostic prediction of biliary tract cancers using droplet digital PCR.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of clinical laboratory analysis"
DOI: 10.1002/jcla.24103
Abstract: OBJECTIVE Biliary tract cancer (BTC) is a rare malignancy and lack of effective diagnostic and prognostic marker. Here, we aimed to investigate the clinical implication of TP53 mutation detection in BTC using droplet digital PCR (ddPCR).… read more here.
Keywords: biliary tract; droplet digital; btc; digital pcr ... See more keywords
Development of the duplex droplet digital PCR for quantitative monitoring of mixed infections of Meloidogyne incognita and M. enterolobii.
Sign Up to like & getrecommendations! Published in 2024 at "Pest management science"
DOI: 10.1002/ps.8553
Abstract: BACKGROUND Root-knot nematodes (RKN, Meloidogyne spp.) are economically significant pests that cause immense damage to a wide range of crops. Among them, M. incognita and M. enterolobii are of particular concern, as their high virulence… read more here.
Keywords: mixed infections; enterolobii; duplex droplet; droplet digital ... See more keywords
Near-Infrared Responsive Droplet for Digital PCR.
Sign Up to like & getrecommendations! Published in 2022 at "Small"
DOI: 10.1002/smll.202107858
Abstract: Digital PCR (dPCR) surpasses the performance of earlier PCR formats because of highly precise, absolute quantification and other unique merits. A simple thermocycling approach and durable microcarrier are of great value for dPCR advancement and… read more here.
Keywords: responsive droplet; near infrared; digital pcr; infrared responsive ... See more keywords
Analysis of miRNA Expression Using Digital PCR and the QuantStudio™ 3D Digital PCR System.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1507-2_13
Abstract: miRNAs are short, single-stranded RNA molecules that function as posttranscriptional regulators of gene expression. miRNAs represent ideal biomarkers since they can also circulate in the bloodstream as well as in other biological fluids such as… read more here.
Keywords: pcr system; analysis; pcr; digital pcr ... See more keywords
Droplet-Based Microfluidics Digital PCR for the Detection of KRAS Mutations.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6734-6_12
Abstract: We demonstrate an accurate and sensitive quantification of mutated KRAS oncogene in genomic DNA, using droplet-based microfluidics and digital PCR. read more here.
Keywords: detection kras; based microfluidics; microfluidics digital; digital pcr ... See more keywords