Low-copy transgene detection using nested digital PCR for gene doping control.
Sign Up to like & getrecommendations! Published in 2021 at "Drug testing and analysis"
DOI: 10.1002/dta.3173
Abstract: Gene doping is prohibited for fair competition in human and horse sports. One style of gene doping is the administration of an exogeneous gene, called a transgene, to postnatal humans and horses. Although many transgene… read more here.
Keywords: detection; pcr; gene doping; digital pcr ... See more keywords
A poly(dimethylsiloxane) microfluidic sheet reversibly adhered on a glass plate for creation of emulsion droplets for droplet digital PCR
Sign Up to like & getrecommendations! Published in 2017 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201600309
Abstract: A PDMS microfluidic chip with T‐junction channel geometry, two inlet reservoirs, and one outlet reservoir was reversibly adhered on a glass plate through the viscoelastic properties of PDMS. This formed a detachable microfluidic device for… read more here.
Keywords: glass plate; creation; droplet digital; glass ... See more keywords
Design of a digital‐PCR assay to quantify fragmented human mitochondrial DNA
Sign Up to like & getrecommendations! Published in 2021 at "Environmental and Molecular Mutagenesis"
DOI: 10.1002/em.22449
Abstract: Digital PCR (dPCR) has been adapted to quantify the proportion of mitochondrial DNA (mtDNA) molecules without and with double‐strand DNA breaks (DSBs). This is based on a break‐apart approach of two differentially labeled target sequences… read more here.
Keywords: molecule; mtdna; dna; digital pcr ... See more keywords
Quantitative detections of TP53 gene mutations improve the diagnosis and prognostic prediction of biliary tract cancers using droplet digital PCR.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of clinical laboratory analysis"
DOI: 10.1002/jcla.24103
Abstract: OBJECTIVE Biliary tract cancer (BTC) is a rare malignancy and lack of effective diagnostic and prognostic marker. Here, we aimed to investigate the clinical implication of TP53 mutation detection in BTC using droplet digital PCR (ddPCR).… read more here.
Keywords: biliary tract; droplet digital; btc; digital pcr ... See more keywords
Near-Infrared Responsive Droplet for Digital PCR.
Sign Up to like & getrecommendations! Published in 2022 at "Small"
DOI: 10.1002/smll.202107858
Abstract: Digital PCR (dPCR) surpasses the performance of earlier PCR formats because of highly precise, absolute quantification and other unique merits. A simple thermocycling approach and durable microcarrier are of great value for dPCR advancement and… read more here.
Keywords: responsive droplet; near infrared; digital pcr; infrared responsive ... See more keywords
Analysis of miRNA Expression Using Digital PCR and the QuantStudio™ 3D Digital PCR System.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1507-2_13
Abstract: miRNAs are short, single-stranded RNA molecules that function as posttranscriptional regulators of gene expression. miRNAs represent ideal biomarkers since they can also circulate in the bloodstream as well as in other biological fluids such as… read more here.
Keywords: pcr system; analysis; pcr; digital pcr ... See more keywords
Droplet-Based Microfluidics Digital PCR for the Detection of KRAS Mutations.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6734-6_12
Abstract: We demonstrate an accurate and sensitive quantification of mutated KRAS oncogene in genomic DNA, using droplet-based microfluidics and digital PCR. read more here.
Keywords: detection kras; based microfluidics; microfluidics digital; digital pcr ... See more keywords
Quantitative Real-Time PCR and Digital PCR to Evaluate Residual Quantity of HAV in Experimentally Depurated Mussels.
Sign Up to like & getrecommendations! Published in 2021 at "Food and environmental virology"
DOI: 10.1007/s12560-021-09470-4
Abstract: Kinetics of hepatitis A virus (HAV) accumulation and depuration from mussels (Mytilus galloprovincialis) was studied in an experimental depuration system. Different parameters likely to influence the rate of virus accumulation and elimination were evaluated. Analyses… read more here.
Keywords: hav; real time; depuration; digital pcr ... See more keywords
A self-digitization chip integrated with hydration layer for low-cost and robust digital PCR.
Sign Up to like & getrecommendations! Published in 2019 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2018.12.029
Abstract: We present a self-discretization and zero-water-loss microfluidic digital PCR (dPCR) device to enable low-cost and robust quantitative nucleic acid assays. In this device, a thin void is integrated beneath the reaction chamber array. By utilizing… read more here.
Keywords: cost robust; low cost; digital pcr; hydration ... See more keywords
Fully automatic integrated continuous-flow digital PCR device for absolute DNA quantification.
Sign Up to like & getrecommendations! Published in 2020 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2020.05.044
Abstract: Existing digital polymerase chain reaction (PCR) devices consist of multiple independent devices, such as a droplet generator, a PCR thermocycler, and a droplet reader, which have the disadvantages of low integration, complex equipment structure, and… read more here.
Keywords: pcr device; fully automatic; pcr; digital pcr ... See more keywords
Digital PCR Method for Detection and Quantification of Specific Antimicrobial Drug-Resistance Mutations in Human Cytomegalovirus.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2020.113864
Abstract: Antimicrobial drug resistance is one of the biggest threats to human health worldwide. Timely detection and quantification of infectious agents and their susceptibility to antimicrobial drugs are crucial for efficient management of resistance to antiviral… read more here.
Keywords: drug resistance; quantification; digital pcr; resistance ... See more keywords