An improved digital polymerase chain reaction protocol to capture low‐copy KRAS mutations in plasma cell‐free DNA by resolving ‘subsampling’ issues
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Oncology"
DOI: 10.1002/1878-0261.12110
Abstract: Genetic alterations responsible for the initiation of cancer may serve as immediate biomarkers for early diagnosis. Plasma levels of cell‐free DNA (cfDNA) in patients with cancer are higher than those in healthy individuals; however, the… read more here.
Keywords: resolving subsampling; free dna; cell free; chain reaction ... See more keywords
Easy-to-Operate Co-flow Step Emulsification Device for Droplet Digital Polymerase Chain Reaction.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c04983
Abstract: Digital polymerase chain reaction (PCR) plays important roles in the detection and quantification of nucleic acid targets, while there still remain challenges including high cost, complex operation, and low integration of the instrumental system. Here,… read more here.
Keywords: step; chain reaction; polymerase chain; droplet ... See more keywords
Evaluating Droplet Digital Polymerase Chain Reaction for the Quantification of Human Genomic DNA: Lifting the Traceability Fog.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b00240
Abstract: Digital polymerase chain reaction (dPCR) end point platforms directly estimate the number of DNA target copies per reaction partition, λ, where the partitions are fixed-location chambers (cdPCR) or aqueous droplets floating in oil (ddPCR). For… read more here.
Keywords: traceability; chain reaction; reaction; polymerase chain ... See more keywords
Digital Polymerase Chain Reaction for Assessment of Mutant Mitochondrial Carry-over after Nuclear Transfer for In Vitro Fertilization.
Sign Up to like & getrecommendations! Published in 2021 at "Clinical chemistry"
DOI: 10.1093/clinchem/hvab021
Abstract: BACKGROUND The quantification of mitochondrial DNA heteroplasmy for the diagnosis of mitochondrial disease or after mitochondrial donation, is performed mainly using next-generation sequencing strategies (NGS). Digital PCR (dPCR) has the potential to offer an accurate… read more here.
Keywords: mutation load; chain reaction; polymerase chain; digital polymerase ... See more keywords
Viability droplet digital polymerase chain reaction accurately enumerates probiotics and provides insight into damage experienced during storage
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.966264
Abstract: Probiotics are typically enumerated by agar plate counting (PC) techniques. PC has several limitations including poor specificity, high variability, inability to enumerate dead cells, viable but non-culturable cells and cells in complex matrices. Viability droplet… read more here.
Keywords: viability droplet; chain reaction; polymerase chain; digital polymerase ... See more keywords