GeoMx™ RNA Assay: High Multiplex, Digital, Spatial Analysis of RNA in FFPE Tissue.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0623-0_21
Abstract: RNA in situ hybridization (ISH) is a widely used technique for the localization of mRNA in tissues. Limitations to traditional ISH include the number of targets that can be analyzed concurrently and the ability for… read more here.
Keywords: digital spatial; rna assay; assay high; rna ... See more keywords
Multiplex digital spatial profiling of proteins and RNA in fixed tissue
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DOI: 10.1038/s41587-020-0472-9
Abstract: Digital Spatial Profiling (DSP) is a method for highly multiplex spatial profiling of proteins or RNAs suitable for use on formalin-fixed, paraffin-embedded (FFPE) samples. The approach relies on (1) multiplexed readout of proteins or RNAs… read more here.
Keywords: digital spatial; proteins rna; rna fixed; profiling proteins ... See more keywords
43 Highly multiplexed digital spatial profiling of the tumor microenvironment of non-small-cell lung cancer (NSCLC)
Sign Up to like & getrecommendations! Published in 2020 at "Journal for ImmunoTherapy of Cancer"
DOI: 10.1136/jitc-2020-sitc2020.0043
Abstract: Background Profiling the tumour microenvironment (TME) has been informative in understanding the underlying tumour-immune interactions. Multiplex immunohistochemistry(mIHC) coupled with molecular barcoding technologies have revealed greater insights into the TME. Methods In this study, we utilised… read more here.
Keywords: microenvironment; digital spatial; multiplexed digital; highly multiplexed ... See more keywords
305 Technical considerations for normalizing digital spatial profiling data with multiple within-patient samples
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DOI: 10.1136/jitc-2020-sitc2020.0305
Abstract: Background NanoString’s GeoMx Digital Spatial Profiling (DSP) technology enables profiling of gene or protein expression from fresh or archival tissues. Specific regions of interest (ROIs) are identified via fluorescently labeled visualization markers. Within a given… read more here.
Keywords: digital spatial; considerations normalizing; expression; spatial profiling ... See more keywords
Abstract 6778: Spatial analysis of genomic signatures on colorectal cancer pathogenesis using the GeoMx® Digital Spatial Profiler
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DOI: 10.1158/1538-7445.am2023-6778
Abstract: Background: The development of colorectal cancer (CRC) is a complex process, involving multiple sequential molecular aberrations that contribute to disease progression. Understanding the immunopathogenesis of CRC particularly within the spatial context of tumor microenvironment (TME)… read more here.
Keywords: geomx digital; analysis; colorectal cancer; digital spatial ... See more keywords
Digital Spatial Profiling of Individual Glomeruli From Patients With Anti-Neutrophil Cytoplasmic Autoantibody-Associated Glomerulonephritis
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DOI: 10.3389/fimmu.2022.831253
Abstract: We previously showed that the rupture of Bowman’s capsule (BC) promotes the progression of crescentic glomerulonephritis by enhancing the entry of CD8+ T cells into the glomeruli. In the present study, we utilized digital spatial… read more here.
Keywords: glomeruli; neutrophil cytoplasmic; spatial profiling; digital spatial ... See more keywords
An integrated model of acinar to ductal metaplasia-related N7-methyladenosine regulators predicts prognosis and immunotherapy in pancreatic carcinoma based on digital spatial profiling
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DOI: 10.3389/fimmu.2022.961457
Abstract: Acinar-to-ductal metaplasia (ADM) is a recently recognized, yet less well-studied, precursor lesion of pancreatic ductal adenocarcinoma (PDAC) developed in the setting of chronic pancreatitis. Through digital spatial mRNA profiling, we compared ADM and adjacent PDAC… read more here.
Keywords: immune cell; ductal metaplasia; digital spatial; model ... See more keywords
Highly Multiplexed Digital Spatial Profiling of the Tumor Microenvironment of Head and Neck Squamous Cell Carcinoma Patients
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DOI: 10.3389/fonc.2020.607349
Abstract: Background Immune checkpoint inhibitors (ICI) have shown durable and long-term benefits in a subset of head and neck squamous cell carcinoma (HNSCC) patients. To identify patient-responders from non-responders, biomarkers are needed which are predictive of… read more here.
Keywords: neck squamous; digital spatial; cell carcinoma; squamous cell ... See more keywords
Short Digital Spatial Memory Test Detects Impairment in Alzheimer’s Disease and Mild Cognitive Impairment
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DOI: 10.3390/brainsci11101350
Abstract: Background: Impairment in navigation abilities and object location memory are often seen in early-stage Alzheimer’s Disease (AD), yet these constructs are not included in standard neuropsychological assessment. We investigated the differential ability of a short… read more here.
Keywords: digital spatial; memory test; short digital; impairment ... See more keywords