Measurement of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation.
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DOI: 10.1007/978-1-4939-6625-7_19
Abstract: The fluorogenic probe dihydroethidium (DHE) is widely used for detecting intracellular superoxide. DHE oxidation by superoxide generates specifically the compound 2-hydroxyethidium (2-E+OH), so that 2-E+OH detection confers specificity to superoxide assessment among many other reactive… read more here.
Keywords: hplc; oxidation; dihydroethidium; superoxide ... See more keywords