Two-Dimensional Gel Electrophoresis: A Reference Protocol.
Sign Up to like & getrecommendations! Published in 2017 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-3-319-52479-5_13
Abstract: Two-dimensional gel electrophoresis (2DE) has been a mainstay of proteomic techniques for more than four decades. It was even in use for several years before the term proteomics was actually coined in the early 1990s.… read more here.
Keywords: dimensional gel; reference protocol; gel electrophoresis; electrophoresis reference ... See more keywords
Two-Dimensional Gel Electrophoresis of DNA Replication Intermediates in Schizosaccharomyces pombe.
Sign Up to like & getrecommendations! Published in 2018 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot092007
Abstract: Two-dimensional gel electrophoresis allows direct detection of DNA replication and recombination intermediates in preparations of total genomic DNA. This technique is widely used to identify replication origins in the yeast genome and is based on… read more here.
Keywords: dimensional gel; gel electrophoresis; dna; two dimensional ... See more keywords
Optimization of protein extraction and two-dimensional gel electrophoresis profiles for the identification of Cordyceps sinensis and other similar species
Sign Up to like & getrecommendations! Published in 2018 at "PLoS ONE"
DOI: 10.1371/journal.pone.0202779
Abstract: Given that Chinese materia medica (CMM) is expensive and rare, people take tremendous risk to adulterate and falsify Cordyceps sinensis with counterfeit species with similar morphological features. It is thus essential to develop new methods… read more here.
Keywords: sinensis; dimensional gel; gel electrophoresis; electrophoresis profiles ... See more keywords
Innovating the Concept and Practice of Two-Dimensional Gel Electrophoresis in the Analysis of Proteomes at the Proteoform Level
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DOI: 10.3390/proteomes7040036
Abstract: Two-dimensional gel electrophoresis (2DE) is an important and well-established technical platform enabling extensive top-down proteomic analysis. However, the long-held but now largely outdated conventional concepts of 2DE have clearly impacted its application to in-depth investigations… read more here.
Keywords: concept; dimensional gel; gel electrophoresis; analysis ... See more keywords
What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World?
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DOI: 10.3390/proteomes8030017
Abstract: Two-dimensional gel electrophoresis was instrumental in the birth of proteomics in the late 1980s. However, it is now often considered as an outdated technique for proteomics—a thing of the past. Although this opinion may be… read more here.
Keywords: dimensional gel; gel electrophoresis; based proteomics; two dimensional ... See more keywords