Real-time monitoring of D-Ala-D-Ala dipeptidase activity of VanX in living bacteria by isothermal titration calorimetry.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2019.05.002
Abstract: The d,d-dipeptidase enzyme VanX is the main cause of vancomycin resistance in gram-positive bacteria because of hydrolysis of the D-Ala-D-Ala dipeptide used in cell-wall biosynthesis. Continuous assay of VanX has proven challenging due to lack… read more here.
Keywords: ala ala; dipeptidase; titration calorimetry; isothermal titration ... See more keywords
Structure of human DPEP3 in complex with the SC-003 antibody Fab fragment reveals basis for lack of dipeptidase activity.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of structural biology"
DOI: 10.1016/j.jsb.2020.107512
Abstract: Dipeptidase 3 (DPEP3) is one of three glycosylphosphatidylinositol-anchored metallopeptidases potentially involved in the hydrolytic metabolism of dipeptides. While its exact biological function is not clear, DPEP3 expression is normally limited to testis , but can… read more here.
Keywords: fab fragment; dipeptidase; dpep3; antibody ... See more keywords
Lysis of a Lactococcus lactis Dipeptidase Mutant and Rescue by Mutation in the Pleiotropic Regulator CodY
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DOI: 10.1128/aem.02937-19
Abstract: Precise control of peptidoglycan synthesis is essential in Gram-positive bacteria for maintaining cell shape and integrity as well as resisting stresses. Although neither the dipeptidase PepV nor alanine is essential for L. lactis MG1363, adequate… read more here.
Keywords: dipeptidase; synthesis; cody; cell ... See more keywords