Complex Evolutionary History of Translation Elongation Factor 2 and Diphthamide Biosynthesis in Archaea and Parabasalids
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DOI: 10.1093/gbe/evy154
Abstract: Abstract Diphthamide is a modified histidine residue which is uniquely present in archaeal and eukaryotic elongation factor 2 (EF-2), an essential GTPase responsible for catalyzing the coordinated translocation of tRNA and mRNA through the ribosome.… read more here.
Keywords: diphthamide biosynthesis; biosynthesis; elongation factor;
Organometallic and radical intermediates reveal mechanism of diphthamide biosynthesis
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DOI: 10.1126/science.aao6595
Abstract: A quick freeze shows an enzyme's secrets Organic radicals are chemically useful in enzymatic reactions but are often hard to observe, owing to their short lifetimes. Dong et al. used rapid freeze-quench methods to trap… read more here.
Keywords: carbon; carbon bond; sam; diphthamide biosynthesis ... See more keywords
Identification of the transcription factor Miz1 as an essential regulator of diphthamide biosynthesis using a CRISPR-mediated genome-wide screen
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DOI: 10.1371/journal.pgen.1009068
Abstract: Diphthamide is a unique post-translationally modified histidine residue (His715 in all mammals) found only in eukaryotic elongation factor-2 (eEF-2). The biosynthesis of diphthamide represents one of the most complex modifications, executed by protein factors conserved… read more here.
Keywords: transcription factor; diphthamide biosynthesis; biosynthesis;