Diphtheria toxin-mediated transposon-driven poly (A)-trapping efficiently disrupts transcriptionally silent genes in embryonic stem cells.
Sign Up to like & getrecommendations! Published in 2020 at "Genesis"
DOI: 10.1002/dvg.23386
Abstract: Random gene trapping is the application of insertional mutagenesis techniques that are conventionally used to inactivate protein-coding genes in mouse embryonic stem (ES) cells. Transcriptionally silent genes are not effectively targeted by conventional random gene… read more here.
Keywords: vector; transcriptionally silent; embryonic stem; stem cells ... See more keywords
Treatment of MCPT8DTR mice with high‐ or low‐dose diphtheria toxin leads to differential depletion of basophils and granulocyte‐macrophage progenitors
Sign Up to like & getrecommendations! Published in 2018 at "European Journal of Immunology"
DOI: 10.1002/eji.201747351
Abstract: Basophils have been recently recognized to play important roles in type 2 immune responses during allergies and parasitic infection, largely due to the development of novel tools for the in vivo study of these cells.… read more here.
Keywords: mcpt8dtr; macrophage progenitors; eosinophils neutrophils; mcpt8dtr mice ... See more keywords
Novel Microglia Depletion Systems: A Genetic Approach Utilizing Conditional Diphtheria Toxin Receptor Expression and a Pharmacological Model Based on the Blocking of Macrophage Colony-Stimulating Factor 1 Receptor.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9658-2_16
Abstract: Microglia are the main population of macrophage residing in the central nervous system (CNS). Depletion experiments gave important insights into the physiology and function of microglia in healthy and diseased CNS. Ablation of microglia can be… read more here.
Keywords: model; macrophage; receptor; diphtheria toxin ... See more keywords
NMR study of mutations of glycine-52 of the catalytic domain of diphtheria toxin.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of pharmaceutical and biomedical analysis"
DOI: 10.1016/j.jpba.2017.11.056
Abstract: Cross-reacting-material 197 (CRM197) is a naturally occurring non-toxic mutant of diphtheria toxin (DT) that is one of the few carrier protein used in the manufacture of polysaccharide vaccines targeting bacterial pathogens such as Neisseria meningitidis,… read more here.
Keywords: domain diphtheria; catalytic domain; domain; diphtheria toxin ... See more keywords
Structures of distant diphtheria toxin homologs reveal functional determinants of an evolutionarily conserved toxin scaffold
Sign Up to like & getrecommendations! Published in 2022 at "Communications Biology"
DOI: 10.1038/s42003-022-03333-9
Abstract: Diphtheria toxin (DT) is the archetype for bacterial exotoxins implicated in human diseases and has played a central role in defining the field of toxinology since its discovery in 1888. Despite being one of the… read more here.
Keywords: functional determinants; diphtheria toxin; structures distant; toxin ... See more keywords
Corynebacterium diphtheriae: Diphtheria Toxin, the tox Operon, and Its Regulation by Fe2+ Activation of apo-DtxR.
Sign Up to like & getrecommendations! Published in 2019 at "Microbiology spectrum"
DOI: 10.1128/microbiolspec.gpp3-0063-2019
Abstract: Diphtheria is one of the most well studied of all the bacterial infectious diseases. These milestone studies of toxigenic Corynebacterium diphtheriae along with its primary virulence determinant, diphtheria toxin, have established the paradigm for the… read more here.
Keywords: activation apo; regulation; corynebacterium diphtheriae; diphtheria toxin ... See more keywords
Structure–Activity Relationship of the Dimeric and Oligomeric Forms of a Cytotoxic Biotherapeutic Based on Diphtheria Toxin
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DOI: 10.3390/biom12081111
Abstract: Protein aggregation is a well-recognized problem in industrial preparation, including biotherapeutics. These low-energy states constantly compete with a native-like conformation, which is more pronounced in the case of macromolecules of low stability in the solution.… read more here.
Keywords: structure activity; dimeric oligomeric; diphtheria toxin;
Determination of Diphtheria Toxin in Bacterial Cultures by Enzyme Immunoassay
Sign Up to like & getrecommendations! Published in 2022 at "Diagnostics"
DOI: 10.3390/diagnostics12092204
Abstract: Since diphtheria toxin (DT) is the main virulence factor of Corynebacterium diphtheriae and C. ulcerans, the detection of DT in corynebacterial cultures is of utmost importance in the laboratory diagnosis of diphtheria. The need to… read more here.
Keywords: toxin bacterial; diphtheria; diphtheria toxin; determination diphtheria ... See more keywords