Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION™ nanopore sequencer
Sign Up to like & getrecommendations! Published in 2019 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.12590
Abstract: Rapid identification of bacterial pathogens is crucial for appropriate and adequate antibiotic treatment, which significantly improves patient outcomes. 16S ribosomal RNA (rRNA) gene amplicon sequencing has proven to be a powerful strategy for diagnosing bacterial… read more here.
Keywords: identification; 16s rrna; minion; direct pcr ... See more keywords
A Novel Direct PCR Lysis Buffer Can Improve PCR from Meat Matrices
Sign Up to like & getrecommendations! Published in 2018 at "Food Analytical Methods"
DOI: 10.1007/s12161-018-1342-7
Abstract: Molecular technologies based on PCR have been widely used in many biological analysis fields, especially for genetic analysis and DNA barcoding. In this study, a rapid DNA lysis liquid was formulated without any purification step… read more here.
Keywords: direct pcr; dna; pcr; meat ... See more keywords
A practical study on direct PCR amplification using the GlobalFiler™ PCR Amplification Kit on human bloodstains collected with microFLOQ™ Direct swabs.
Sign Up to like & getrecommendations! Published in 2019 at "Forensic science international"
DOI: 10.1016/j.forsciint.2019.04.018
Abstract: Rapid DNA profiling of casework samples is a powerful tool that can support law enforcement agencies in the quick apprehension of perpetrators before they re-offend or escape the jurisdiction. This present study evaluated the feasibility… read more here.
Keywords: pcr amplification; direct pcr; dna; microfloq direct ... See more keywords
Direct STR typing from fired and unfired bullet casings.
Sign Up to like & getrecommendations! Published in 2019 at "Forensic science international"
DOI: 10.1016/j.forsciint.2019.05.037
Abstract: Cartridges, bullets, and casings (CBCs) are commonly recovered after shooting incidents and could provide valuable DNA information from touch DNA that has been left behind during handling of bullets and loading of guns. Direct PCR,… read more here.
Keywords: dna; touch dna; bullet casings; direct pcr ... See more keywords
Automating direct-to-PCR for disaster victim identification
Sign Up to like & getrecommendations! Published in 2019 at "Australian Journal of Forensic Sciences"
DOI: 10.1080/00450618.2019.1569145
Abstract: ABSTRACT Direct-to-PCR methodology adds samples directly to PCR tubes offering gains in efficiency and sensitivity. The approach has been applied to a variety of biological sources including blood, saliva, tissue, hair and nail. We added… read more here.
Keywords: disaster victim; victim identification; methodology; direct pcr ... See more keywords
Review of direct PCR and Rapid DNA approaches to streamline sexual assault kit testing.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of forensic sciences"
DOI: 10.1111/1556-4029.15044
Abstract: Crime laboratories have been faced with large casework backlogs due to lengthy processing times, limited resources and scientists, and rising crime rates. Evidence related to sexual assault crimes, specifically sexual assault kits (SAKs), heavily contribute… read more here.
Keywords: dna; pcr rapid; sexual assault; direct pcr ... See more keywords
Evaluation of DNA extraction methods and direct PCR in metabarcoding of mock and marine bacterial communities
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2023.1151907
Abstract: Recent advances in new molecular biology methods and next-generation sequencing (NGS) technologies have revolutionized metabarcoding studies investigating complex microbial communities from various environments. The inevitable first step in sample preparation is DNA extraction which introduces… read more here.
Keywords: mock; dna extraction; extraction; direct pcr ... See more keywords
Clinical Evaluation of Nasopharyngeal, Oropharyngeal, Nasal Swabs, and Saliva for the Detection of SARS-CoV-2 by Direct RT-PCR
Sign Up to like & getrecommendations! Published in 2022 at "Diagnostics"
DOI: 10.3390/diagnostics12051091
Abstract: Nasopharyngeal swab (NPS) and oropharyngeal swab (OPS) are the most widely used upper respiratory tract specimens for diagnosis of SARS-CoV-2 using RT-qPCR. In contrast, nasal swab (NS) and saliva (SS), recently recommended by the WHO,… read more here.
Keywords: rna extraction; detection sars; pcr; direct pcr ... See more keywords