Phage Enzyme-Assisted Direct In Vivo DNA Assembly in Multiple Microorganisms.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00529
Abstract: The assembly of DNA fragments is extremely important for molecular biology. Increasing numbers of studies have focused on streamlining the laborious and costly protocols via in vivo DNA assembly. However, the existing methods were mainly… read more here.
Keywords: vivo dna; dna; direct vivo; dna assembly ... See more keywords
AAV-mediated direct in vivo CRISPR screen identifies functional suppressors in glioblastoma
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DOI: 10.1038/nn.4620
Abstract: A causative understanding of genetic factors that regulate glioblastoma pathogenesis is of central importance. Here we developed an adeno-associated virus–mediated, autochthonous genetic CRISPR screen in glioblastoma. Stereotaxic delivery of a virus library targeting genes commonly… read more here.
Keywords: mediated direct; crispr; direct vivo; aav mediated ... See more keywords
Direct In Vivo Analysis of CBD- and THC-Acid Cannabinoids and Classification of Cannabis Cultivars Using SpiderMass
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DOI: 10.3390/metabo12060480
Abstract: In recent years, cannabis and hemp-based products have become increasingly popular for recreational use, edibles, beverages, health care products, and medicines. The rapid detection and differentiation of phytocannabinoids is, therefore, essential to assess the potency… read more here.
Keywords: cannabis cultivars; vivo analysis; direct vivo; spidermass ... See more keywords