Directed Evolution Enables Simultaneous Controlled Release of Multiple Therapeutic Proteins from Biopolymer‐Based Hydrogels
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Materials"
DOI: 10.1002/adma.202202612
Abstract: With the advent of increasingly complex combination strategies of biologics, independent control over their delivery is the key to their efficacy; however, current approaches are hindered by the limited independent tunability of their release rates.… read more here.
Keywords: multiple therapeutic; therapeutic proteins; controlled release; release ... See more keywords
From directed evolution to computational enzyme engineering—A review
Sign Up to like & getrecommendations! Published in 2019 at "AIChE Journal"
DOI: 10.1002/aic.16847
Abstract: Funding information Division of Chemical, Bioengineering, Environmental, and Transport Systems, Grant/ Award Number: CBET-1703274 Abstract Nature relies on a wide range of enzymes with specific biocatalytic roles to carry out much of the chemistry needed… read more here.
Keywords: computational enzyme; engineering; chemistry; evolution computational ... See more keywords
Directed evolution of polypropylene and polystyrene binding peptides
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26481
Abstract: Surface functionalization of biological inert polymers (e.g., polypropylene PP; polystyrene PS) with material binding peptides facilitates an efficient immobilization of enzymes, bioactive peptides or antigens at ambient temperature in water. The developed robust directed evolution… read more here.
Keywords: binding peptides; evolution; lci; polypropylene polystyrene ... See more keywords
Enzyme‐mediated hydrogel encapsulation of single cells for high‐throughput screening and directed evolution of oxidoreductases
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27002
Abstract: Directed evolution of oxidoreductases to improve their catalytic properties is being ardently pursued in the industrial, biotechnological, and biopharma sectors. Hampering this pursuit are current enzyme screening methods that are limited in terms of throughput,… read more here.
Keywords: hydrogel; evolution; evolution oxidoreductases; gox ... See more keywords
Directed Evolution of a Cp*RhIII‐Linked Biohybrid Catalyst Based on a Screening Platform with Affinity Purification
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DOI: 10.1002/cbic.202000681
Abstract: Directed evolution of Cp*RhIII‐linked nitrobindin (NB), a biohybrid catalyst, was performed based on an in vitro screening approach. A key aspect of this effort was the establishment of a high‐throughput screening (HTS) platform that involves an… read more here.
Keywords: rhiii linked; evolution; evolution rhiii; platform ... See more keywords
Making Enzymes Suitable for Organic Chemistry by Rational Protein Design
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DOI: 10.1002/cbic.202200049
Abstract: This review outlines recent developments in protein engineering of stereo‐ and regioselective enzymes, which are of prime interest in organic and pharmaceutical chemistry as well as biotechnology. The widespread application of enzymes was hampered for… read more here.
Keywords: chemistry; making enzymes; design; enzymes suitable ... See more keywords
Directed Evolution of Proteins Based on Mutational Scanning.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7366-8_6
Abstract: Directed evolution has emerged as one of the most effective protein engineering methods in basic research as well as in applications in synthetic organic chemistry and biotechnology. The successful engineering of protein activity, allostery, binding… read more here.
Keywords: evolution proteins; mutational scanning; chemistry; directed evolution ... See more keywords
[Directed evolution in drug and antibody development : From the Nobel Prize to broad clinical application].
Sign Up to like & getrecommendations! Published in 2019 at "Der Internist"
DOI: 10.1007/s00108-019-00675-1
Abstract: Combinatorial procedures have become established in recent years as alternatives to rational design in drug research, particularly when no structural information is available. This article presents the principle that was originally developed by three scientists… read more here.
Keywords: drug; application; directed evolution; evolution drug ... See more keywords
Enhanced activity and substrate tolerance of 7α-hydroxysteroid dehydrogenase by directed evolution for 7-ketolithocholic acid production
Sign Up to like & getrecommendations! Published in 2019 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-019-09668-4
Abstract: Abstract7-Ketolithocholic acid (7-KLCA) is an important intermediate for the synthesis of ursodeoxycholic acid (UDCA). UDCA is the main effective component of bear bile powder that is used in traditional Chinese medicine for the treatment of… read more here.
Keywords: tolerance; ketolithocholic acid; directed evolution; hydroxysteroid dehydrogenase ... See more keywords
Enhanced the catalytic efficiency and thermostability of maltooligosyltrehalose synthase from Arthrobacter ramosus by directed evolution
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical Engineering Journal"
DOI: 10.1016/j.bej.2020.107724
Abstract: Abstract Maltooligosyltrehalose synthase (MTSase) is a key enzyme in the preparation of trehalose. Increasing interest in trehalose requires further improvement in the catalytic performance of MTSase in order to increase trehalose yield, but so far,… read more here.
Keywords: arthrobacter ramosus; maltooligosyltrehalose synthase; mtsase; directed evolution ... See more keywords
Hot spots-making directed evolution easier.
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology advances"
DOI: 10.1016/j.biotechadv.2022.107926
Abstract: Directed evolution has emerged as a powerful strategy to engineer various properties of proteins. Traditional methods to construct libraries such as error-prone PCR and DNA shuffling commonly produce large, relatively inefficient libraries. In the absence… read more here.
Keywords: spots making; hot spots; evolution easier; making directed ... See more keywords