Facile Site-Directed Mutagenesis of Large Constructs Using Gibson Isothermal DNA Assembly.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6472-7_24
Abstract: Site-directed mutagenesis is a commonly used molecular biology technique to manipulate biological sequences, and is especially useful for studying sequence determinants of enzyme function or designing proteins with improved activity. We describe a strategy using… read more here.
Keywords: using gibson; biology; site directed; directed mutagenesis ... See more keywords
High-Throughput Site-Directed Mutagenesis.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9624-7_13
Abstract: Protein engineering has an array of uses: whether you are studying a disease mutation, removing undesirable sequences, adding stabilizing mutations for structural purposes, or simply dissecting protein function. Protein engineering is almost exclusively performed using… read more here.
Keywords: throughput; site directed; directed mutagenesis; high throughput ... See more keywords
A single digestion, single-stranded oligonucleotide mediated PCR-independent site-directed mutagenesis method
Sign Up to like & getrecommendations! Published in 2020 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-020-10477-3
Abstract: Abstract A PCR-independent in vitro site-directed mutagenesis method was established. Cas12a from Francisella novicida (FnCas12a) linearizes the plasmid with single digestion. T5 exonuclease removes the target nucleotide. A short single- or double-stranded mutagenic oligonucleotide introduces… read more here.
Keywords: mutagenesis; directed mutagenesis; mutagenesis method; site ... See more keywords
Increase of Bacillus badius Phenylalanine dehydrogenase specificity towards phenylalanine substrate by site-directed mutagenesis.
Sign Up to like & getrecommendations! Published in 2017 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2017.10.009
Abstract: Phenylalanine dehydrogenase (PheDH) is a key enzyme in medical diagnostic for determining the amount of phenylalanine to detect phenylketonuria (PKU) disease. However, determination of phenylalanine can be usually disturbed in presence of tyrosine in blood… read more here.
Keywords: directed mutagenesis; phenylalanine dehydrogenase; phenylalanine; site directed ... See more keywords
Increased activity of β-glucuronidase variants produced by site-directed mutagenesis.
Sign Up to like & getrecommendations! Published in 2018 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2017.09.005
Abstract: β-glucuronidase (BGus) is an essential glycosyl hydrolase which has been widely used in biological and biomedical applications. In this paper, we report the construction and screening of nineteen Escherichia coli BGus (EBGus) mutants using site-directed… read more here.
Keywords: directed mutagenesis; site; glucuronidase; site directed ... See more keywords
Enhanced catalytic efficiency and thermostability of glucose isomerase from Thermoanaerobacter ethanolicus via site-directed mutagenesis.
Sign Up to like & getrecommendations! Published in 2021 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2021.109931
Abstract: Glucose isomerase (GI) is a key enzyme in the preparation of high fructose corn syrup (HFCS). In this study, a mutant TEGI-M-L38 M/V137 L (TEGI-M2) of glucose isomerase (TEGI-M) originated from Thermoanaerobacter ethanalicus CCSD1 was… read more here.
Keywords: glucose isomerase; tegi; directed mutagenesis; site directed ... See more keywords
Improvement of catalytic performance of endoglucanase CgEndo from Colletotrichum graminicola by site-directed mutagenesis.
Sign Up to like & getrecommendations! Published in 2021 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2021.109963
Abstract: In order to improve the catalytic efficiency of cellulase for more effective utilization of lignocellulose, a novel endoglucanase (CgEndo) from Colletotrichum graminicola was expressed by Pichia pastoris X33 and modified by site-directed mutagenesis. Two mutants,… read more here.
Keywords: endoglucanase cgendo; directed mutagenesis; endoglucanase; colletotrichum graminicola ... See more keywords
Site-directed mutagenesis of UbiA to promote menaquinone biosynthesis in Elizabethkingia meningoseptica
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DOI: 10.1016/j.procbio.2017.05.002
Abstract: Abstract To increase the menaquinone (MK) content of an Elizabethkingia meningoseptica, site-directed mutagenesis was generated to suppress 4-hydroxybenzoate octaprenyl transferase (UbiA) activity and subsequently blocked the ubiquinone (UQ) biosynthesis pathway. Fourteen conserved residues except L174… read more here.
Keywords: elizabethkingia meningoseptica; site; site directed; directed mutagenesis ... See more keywords
Thermostability Improvement of the d-Allulose 3-Epimerase from Dorea sp. CAG317 by Site-Directed Mutagenesis at the Interface Regions.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.8b01200
Abstract: d-Allulose is a low-calorie sweetener and has broad applications in the food, cosmetics, and pharmaceutical industries. Recently, most studies focus on d-allulose production from d-fructose by d-allulose 3-epimerase (DAEase). However, the major blocker of industrial… read more here.
Keywords: interface regions; directed mutagenesis; thermostability; site directed ... See more keywords
Synthesis of Recombinant P48 of Mycoplasma agalactiae by Site Directed Mutagenesis and its Immunological Characterization
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DOI: 10.1080/10495398.2016.1189926
Abstract: ABSTRACT Contagious agalactia caused by Mycoplasma agalactiae is an economically important disease of sheep and goats and has been prevalent worldwide including India. The present study was undertaken to evaluate the membrane protein P48 of… read more here.
Keywords: mycoplasma agalactiae; directed mutagenesis; recombinant p48; agalactiae ... See more keywords
Substitutions in PBP3 confer resistance to both ampicillin and extended‐spectrum cephalosporins in Haemophilus parainfluenzae as revealed by site‐directed mutagenesis and gene recombinants
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Antimicrobial Chemotherapy"
DOI: 10.1093/jac/dkx157
Abstract: Objectives: To determine the association of amino acid substitutions in PBP3 with &bgr;‐lactam susceptibility in Haemophilus parainfluenzae. Methods: Single and multiple amino acid mutations at positions 385, 511 and 526 were introduced into PBP3 of… read more here.
Keywords: directed mutagenesis; resistance ampicillin; resistance; substitutions pbp3 ... See more keywords