Multiple Site-Directed and Saturation Mutagenesis by the Patch Cloning Method.
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DOI: 10.1007/978-1-4939-6472-7_22
Abstract: Constructing protein-coding genes with desired mutations is a basic step for protein engineering. Herein, we describe a multiple site-directed and saturation mutagenesis method, termed MUPAC. This method has been used to introduce multiple site-directed mutations… read more here.
Keywords: saturation mutagenesis; multiple site; directed saturation; site directed ... See more keywords