Characterization of a Bi-directional Promoter OtrRp Involved in Oxytetracycline Biosynthesis
Sign Up to like & getrecommendations! Published in 2019 at "Current Microbiology"
DOI: 10.1007/s00284-019-01753-1
Abstract: Previous studies identified a MarR (multiple antibiotic resistance regulator) family transcription factor OtrR in the oxytetracycline biosynthetic gene cluster, which regulated the expression of an efflux pump OtrB. The genes otrB and otrR were divergent… read more here.
Keywords: involved oxytetracycline; characterization directional; promoter otrrp; directional promoter ... See more keywords
Golden Gate assembly with a bi-directional promoter (GBid): A simple, scalable method for phage display Fab library creation
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-59745-2
Abstract: Fabs offer an attractive platform for monoclonal antibody discovery/engineering, but library construction can be cumbersome. We report a simple method – Golden Gate assembly with a bi-directional promoter (GBid) – for constructing phage display Fab… read more here.
Keywords: promoter; phage display; gbid; directional promoter ... See more keywords