Identification and characterization of Poly(ADP-ribose) polymerase-1 interacting proteins during development of Dictyostelium discoideum.
Sign Up to like & getrecommendations! Published in 2021 at "Protein expression and purification"
DOI: 10.1016/j.pep.2021.105923
Abstract: Poly(ADP-ribose) polymerase-1 (PARP-1) is a multifunctional protein that is associated with various biological processes like chromatin remodeling, DNA damage, cell death etc. In Dictyostelium discoideum, PARP-1 has also been implicated in cellular differentiation and development.… read more here.
Keywords: protein; poly adp; interacting proteins; discoideum ... See more keywords
Calcineurin Silencing in Dictyostelium discoideum Leads to Cellular Alterations Affecting Mitochondria, Gene Expression, and Oxidative Stress Response.
Sign Up to like & getrecommendations! Published in 2018 at "Protist"
DOI: 10.1016/j.protis.2018.04.004
Abstract: Calcineurin is involved in development and cell differentiation of the social amoeba Dictyostelium discoideum. However, since knockouts of the calcineurin-encoding genes are not possible in D. discoideum it is assumed that the phosphatase also plays… read more here.
Keywords: calcineurin; dictyostelium discoideum; cellular alterations; discoideum ... See more keywords
The social amoeba dictyostelium discoideum rescues paraburkholderia hayleyella, but not P. agricolaris, from interspecific competition.
Sign Up to like & getrecommendations! Published in 2023 at "FEMS microbiology ecology"
DOI: 10.1093/femsec/fiad055
Abstract: Bacterial endosymbionts can provide benefits for their eukaryotic hosts, but it is often unclear if endosymbionts benefit from these relationships. The social amoeba Dictyostelium discoideum associates with three species of Paraburkholderia endosymbionts, including P. agricolaris… read more here.
Keywords: amoeba dictyostelium; social amoeba; agricolaris; competition ... See more keywords
The specificity of Burkholderia symbionts in the social amoeba farming symbiosis: Prevalence, species, genetic and phenotypic diversity
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Ecology"
DOI: 10.1111/mec.14982
Abstract: The establishment of symbioses between eukaryotic hosts and bacterial symbionts in nature is a dynamic process. The formation of such relationships depends on the life history of both partners. Bacterial symbionts of amoebae may have… read more here.
Keywords: burkholderia; symbiosis; burkholderia symbionts; social amoeba ... See more keywords
Investigation of the host transcriptional response to intracellular bacterial infection using Dictyostelium discoideum as a host model
Sign Up to like & getrecommendations! Published in 2019 at "BMC Genomics"
DOI: 10.1186/s12864-019-6269-x
Abstract: BackgroundDuring infection by intracellular pathogens, a highly complex interplay occurs between the infected cell trying to degrade the invader and the pathogen which actively manipulates the host cell to enable survival and proliferation. Many intracellular… read more here.
Keywords: response; infection; model; host ... See more keywords
A recombinant antibody toolbox for Dictyostelium discoideum
Sign Up to like & getrecommendations! Published in 2020 at "BMC Research Notes"
DOI: 10.1186/s13104-020-05048-8
Abstract: The amoeba Dictyostelium discoideum has been a valuable model organism to study numerous facets of eukaryotic cell biology, such as cell motility, cell adhesion, macropinocytosis and phagocytosis, host–pathogen interactions and multicellular development. However, the relative… read more here.
Keywords: recombinant antibody; antibody toolbox; dictyostelium discoideum; toolbox dictyostelium ... See more keywords
Dictyostelium discoideum cells retain nutrients when the cells are about to outgrow their food source
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Cell Science"
DOI: 10.1242/jcs.260107
Abstract: ABSTRACT Dictyostelium discoideum is a unicellular eukaryote that eats bacteria, and eventually outgrows the bacteria. D. discoideum cells accumulate extracellular polyphosphate (polyP), and the polyP concentration increases as the local cell density increases. At high… read more here.
Keywords: cell; discoideum cells; food; dictyostelium discoideum ... See more keywords
Endosomal vesicle fusion machinery is involved with the contractile vacuole in Dictyostelium discoideum.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of cell science"
DOI: 10.1242/jcs.260477
Abstract: Contractile vacuoles (CVs) share common characteristics, such as a requirement for RAB11 and high levels of V-ATPase. These commonalities suggest a conserved evolutionary origin for the CVs with implications for understanding of the last common… read more here.
Keywords: endosomal vesicle; vesicle fusion; machinery; fusion machinery ... See more keywords
Generation of deletions and precise point mutations in Dictyostelium discoideum using the CRISPR nickase
Sign Up to like & getrecommendations! Published in 2019 at "PLoS ONE"
DOI: 10.1371/journal.pone.0224128
Abstract: The CRISPR/Cas9 system enables targeted genome modifications across a range of eukaryotes. Although we have reported that transient introduction of all-in-one vectors that express both Cas9 and sgRNAs can efficiently induce multiple gene knockouts in… read more here.
Keywords: point mutations; dictyostelium discoideum; precise point; discoideum ... See more keywords
Investigating the Role of Translationally Control Tumor Protein in Growth, Development and Differentiation of Dictyostelium discoideum
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2020.00742
Abstract: Translationally controlled tumor protein (TCTP) is a multifunctional protein implicated in various types of cellular processes involving growth and development of an organism. Here, we identified tctp gene in Dictyostelium discoideum and unraveled its function.… read more here.
Keywords: growth development; tumor protein; discoideum; development ... See more keywords
Dictyostelium Dynamin Superfamily GTPases Implicated in Vesicle Trafficking and Host-Pathogen Interactions
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2021.731964
Abstract: The haploid social amoeba Dictyostelium discoideum is a powerful model organism to study vesicle trafficking, motility and migration, cell division, developmental processes, and host cell-pathogen interactions. Dynamin superfamily proteins (DSPs) are large GTPases, which promote… read more here.
Keywords: dynamin superfamily; biology; discoideum; vesicle trafficking ... See more keywords