Discovery and engineering of a 1-butanol biosensor in Saccharomyces cerevisiae.
Sign Up to like & getrecommendations! Published in 2017 at "Bioresource technology"
DOI: 10.1016/j.biortech.2017.06.114
Abstract: The present study aimed to develop a universal methodology for the discovery of biosensors sensitive to particular stresses or metabolites by using a transcriptome analysis, in order to address the need for in vivo biosensors… read more here.
Keywords: discovery engineering; butanol; discovery; butanol biosensor ... See more keywords
Speeding up enzyme discovery and engineering with ultrahigh-throughput methods.
Sign Up to like & getrecommendations! Published in 2018 at "Current opinion in structural biology"
DOI: 10.1016/j.sbi.2017.12.010
Abstract: Exploring the sequence space of enzyme catalysts is ultimately a numbers game. Ultrahigh-throughput screening methods for rapid analysis of millions of variants are therefore increasingly important for investigating sequence-function relationships, searching large metagenomic libraries for… read more here.
Keywords: discovery engineering; throughput; enzyme discovery; ultrahigh throughput ... See more keywords
Discovery and Engineering of Cytochrome P450s for Terpenoid Biosynthesis.
Sign Up to like & getrecommendations! Published in 2019 at "Trends in biotechnology"
DOI: 10.1016/j.tibtech.2018.11.008
Abstract: Terpenoids represent 60% of known natural products, including many drugs and drug candidates, and their biosynthesis is attracting great interest. However, the unknown cytochrome P450s (CYPs) in terpenoid biosynthetic pathways make the heterologous production of… read more here.
Keywords: terpenoid biosynthesis; discovery engineering; biosynthesis; cytochrome p450s ... See more keywords
Discovery and Engineering of the Cocaine Biosynthetic Pathway.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c09091
Abstract: Cocaine, the archetypal tropane alkaloid from the plant genus Erythroxylum, has recently been used clinically as a topical anesthesia of the mucous membranes. Despite this, the key biosynthetic step of the requisite tropane skeleton (methylecgonone)… read more here.
Keywords: engineering cocaine; pathway; tropane; cocaine ... See more keywords
Discovery and engineering of colchicine alkaloid biosynthesis
Sign Up to like & getrecommendations! Published in 2020 at "Nature"
DOI: 10.1038/s41586-020-2546-8
Abstract: Few complete pathways have been established for the biosynthesis of medicinal compounds from plants. Accordingly, many plant-derived therapeutics are isolated directly from medicinal plants or plant cell culture1. A lead example is colchicine, a US… read more here.
Keywords: discovery engineering; biosynthesis; colchicine; pathway ... See more keywords