An Introduction to Methods for Discovery and Functional Analysis of MicroRNAs in Plants.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9042-9_1
Abstract: MicroRNAs play important roles in posttranscriptional regulation of plant development, metabolism, and abiotic stress responses. The recent generation of massive amounts of small RNA sequence data, along with development of bioinformatic tools to identify miRNAs… read more here.
Keywords: discovery functional; micrornas plants; functional analysis; introduction methods ... See more keywords
Discovery of Functional Macrocyclic Peptides by Means of the RaPID System.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9504-2_14
Abstract: Flexizymes, highly flexible tRNA aminoacylation ribozymes, have enabled charging of virtually any amino acid (including non-proteogenic ones) onto tRNA molecules. Coupling to a custom-made in vitro translation system, namely the flexible in vitro translation (FIT)… read more here.
Keywords: rapid system; system; functional macrocyclic; macrocyclic peptides ... See more keywords
A scalable framework for the discovery of functional helicase substrates and helicase-driven regulatory switches
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2209608119
Abstract: Significance Helicases are ubiquitous NTP-dependent enzymes that disrupt nucleic acid (NA) helices and NA–protein interactions. Despite the implication of helicases in many cellular processes and diseases, their target repertoires and determinants of functional specialization often… read more here.
Keywords: framework discovery; helicase substrates; scalable framework; functional helicase ... See more keywords
Accelerating Discovery of Functional Mutant Alleles in Cancer.
Sign Up to like & getrecommendations! Published in 2018 at "Cancer discovery"
DOI: 10.1158/2159-8290.cd-17-0321
Abstract: Most mutations in cancer are rare, which complicates the identification of therapeutically significant mutations and thus limits the clinical impact of genomic profiling in patients with cancer. Here, we analyzed 24,592 cancers including 10,336 prospectively… read more here.
Keywords: mutant alleles; accelerating discovery; discovery functional; functional mutant ... See more keywords
Discovery and functional characterization of neuropeptides in crinoid echinoderms
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Neuroscience"
DOI: 10.3389/fnins.2022.1006594
Abstract: Neuropeptides are one of the largest and most diverse families of signaling molecules in animals and, accordingly, they regulate many physiological processes and behaviors. Genome and transcriptome sequencing has enabled the identification of genes encoding… read more here.
Keywords: functional characterization; crinoid echinoderms; characterization neuropeptides; discovery functional ... See more keywords
Discovery and Functional Evaluation of Antimicrobials
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DOI: 10.3390/antibiotics10070765
Abstract: Microbial infections pose a continuous threat to human health and healthcare systems around the globe [...]. read more here.
Keywords: functional evaluation; discovery functional; evaluation antimicrobials;
Discovery and Functional Characterization of Two Regulatory Variants Underlying Lupus Susceptibility at 2p13.1
Sign Up to like & getrecommendations! Published in 2022 at "Genes"
DOI: 10.3390/genes13061016
Abstract: Genome-wide association studies have identified 2p13.1 as a prominent susceptibility locus for systemic lupus erythematosus (SLE)—a complex, multisystem autoimmune disease. However, the identity of underlying causal variant (s) and molecular mechanisms for increasing disease susceptibility… read more here.
Keywords: functional characterization; two regulatory; susceptibility; analysis ... See more keywords