A cascade strand displacement amplification strategy for highly sensitive and label-free detection of DNA methyltransferase activity
Sign Up to like & getrecommendations! Published in 2021 at "Microchemical Journal"
DOI: 10.1016/j.microc.2020.105775
Abstract: Abstract DNA methyltransferase (MTase) is regarded as a promising biomarker and therapeutic target, which plays an important regulatory role in many biological processes. Here, a cascade strand displacement amplification strategy was explored for highly sensitive… read more here.
Keywords: strand displacement; detection; dna; displacement amplification ... See more keywords
Fluorescent Sensor Based on Magnetic Separation and Strand Displacement Amplification for the Sensitive Detection of Ochratoxin A
Sign Up to like & getrecommendations! Published in 2023 at "ACS Omega"
DOI: 10.1021/acsomega.3c01408
Abstract: Ochratoxin A (OTA) is a common mycotoxin, and it is a significant threat to human health throughout the food chain. In this study, a sensitive and specific fluorescent sensor based on magnetic separation technology combined… read more here.
Keywords: fluorescent sensor; detection; sensor based; displacement amplification ... See more keywords
Multiple displacement amplification as the first step can increase the diagnostic efficiency of preimplantation genetic testing for monogenic disease for β‐thalassemia
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Obstetrics and Gynaecology Research"
DOI: 10.1111/jog.14003
Abstract: To evaluate whether using multiple displacement amplification (MDA) as the first step can increase the diagnostic efficiency of preimplantation genetic testing for monogenic disease (PGT‐M) for β‐thalassemia. read more here.
Keywords: diagnostic efficiency; displacement amplification; multiple displacement; step increase ... See more keywords
Circular RNA Profiling by Illumina Sequencing via Template-Dependent Multiple Displacement Amplification
Sign Up to like & getrecommendations! Published in 2019 at "BioMed Research International"
DOI: 10.1155/2019/2756516
Abstract: Circular RNAs (circRNAs) are newly discovered incipient non-coding RNAs with potential roles in disease progression in living organisms. Significant reports, since their inception, highlight the abundance and putative functional roles of circRNAs in every organism… read more here.
Keywords: template dependent; illumina sequencing; dependent multiple; displacement amplification ... See more keywords
Chimeric DNA byproducts in strand displacement amplification using the T7 replisome
Sign Up to like & getrecommendations! Published in 2022 at "PLoS ONE"
DOI: 10.1371/journal.pone.0273979
Abstract: Recent advances in next generation sequencing technologies enable reading DNA molecules hundreds of kilobases in length and motivate development of DNA amplification methods capable of producing long amplicons. In vivo, DNA replication is performed not… read more here.
Keywords: dna; using replisome; amplification; chimeric dna ... See more keywords
Template-dependent multiple displacement amplification for profiling human circulating RNA.
Sign Up to like & getrecommendations! Published in 2017 at "BioTechniques"
DOI: 10.2144/000114566
Abstract: Multiple displacement amplification (MDA) is widely used in whole-genome/transcriptome amplification. However, template-independent amplification (TIA) in MDA is a commonly observed phenomenon, particularly when using high concentrations of random hexamer primers and extended incubation times. Here,… read more here.
Keywords: template dependent; circulating rna; displacement amplification; multiple displacement ... See more keywords
Comparison of Multiple Displacement Amplification (MDA) and Multiple Annealing and Looping-Based Amplification Cycles (MALBAC) in Limited DNA Sequencing Based on Tube and Droplet
Sign Up to like & getrecommendations! Published in 2020 at "Micromachines"
DOI: 10.3390/mi11070645
Abstract: Whole genome amplification (WGA) is crucial for whole genome sequencing to investigate complex genomic alteration at the single-cell or even single-molecule level. Multiple displacement amplification (MDA) and multiple annealing and looping based amplification cycles (MALBAC)… read more here.
Keywords: mda; displacement amplification; amplification mda; multiple displacement ... See more keywords
Development of Piezo-Driven Compliant Bridge Mechanisms: General Analytical Equations and Optimization of Displacement Amplification
Sign Up to like & getrecommendations! Published in 2017 at "Micromachines"
DOI: 10.3390/mi8080238
Abstract: Compliant bridge mechanisms are frequently utilized to scale micrometer order motions of piezoelectric actuators to levels suitable for desired applications. Analytical equations have previously been specifically developed for two configurations of bridge mechanisms: parallel and… read more here.
Keywords: displacement amplification; analytical equations; compliant bridge; bridge mechanisms ... See more keywords