Articles with "displacement assay" as a keyword



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VEGFR1 domain 2 covalent labeling with horseradish peroxidase: Development of a displacement assay on VEGF.

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Published in 2017 at "Analytical biochemistry"

DOI: 10.1016/j.ab.2017.05.004

Abstract: The VEGFR1 has been shown to play a role in the regulation of angiogenesis, and has therefore been associated to several pathologies. In order to extend our toolbox of screening methods for the identification of… read more here.

Keywords: assay vegf; displacement assay; vegf; vegfr1 domain ... See more keywords
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Amperometric indicator displacement assay for biomarker monitoring: Indirectly sensing strategy for electrochemically inactive sarcosine.

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Published in 2017 at "Talanta"

DOI: 10.1016/j.talanta.2017.03.009

Abstract: Indicator displacement assay plays a fundamental role in the development of chemosensors. We explored here an ingenious yet effective strategy for amperometric assay of electrochemically inactive sarcosine based on an indicator displacement principle, in which… read more here.

Keywords: indicator displacement; electrochemically inactive; displacement assay; indicator ... See more keywords
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Azocalixarene-Based Supramolecular System for the Detection of Paraquat via an Improved Indicator Displacement Assay.

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Published in 2022 at "Journal of agricultural and food chemistry"

DOI: 10.1021/acs.jafc.2c06051

Abstract: In view of the lethal toxicity of paraquat (PQ) on human health, herein, a simple indicator displacement assay (IDA) based on an azo-modified calixarene host (azoCX[4]) and a fluorophore guest (p-DPD) were elaborately constructed for… read more here.

Keywords: detection; displacement assay; probe; indicator displacement ... See more keywords
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Fluorescence “Turn-On” Indicator Displacement Assay-Based Sensing of Nitroexplosive 2,4,6-Trinitrophenol in Aqueous Media via a Polyelectrolyte and Dye Complex

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Published in 2017 at "ACS Omega"

DOI: 10.1021/acsomega.7b00765

Abstract: A water-soluble nonfluorescent cationic conjugated polyelectrolyte poly(1,1′-((1,4-phenylenebis(oxy))bis(propane-3,1-diyl))bis(pyridin-1-ium)bromide) (PPPy) was specifically synthesized via an economical method of oxidative coupling polymerization in high yields. PPPy selectively recognized nitroexplosive picric acid (PA) by fluorescence “turn-on” in the presence… read more here.

Keywords: indicator displacement; displacement assay; fluorescence; fluorescence turn ... See more keywords
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A 'light-up' intercalator displacement assay for detection of triplex DNA stabilizers.

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Published in 2020 at "Chemical communications"

DOI: 10.1039/c9cc08817b

Abstract: Here, we developed a coralyne-based, 'light-up' intercalator displacement assay to identify molecular stabilizers of triplex DNA using a sequence from a chromosomal breakpoint hotspot in the human c-MYC oncogene. Its potential to identify triplex DNA… read more here.

Keywords: intercalator displacement; displacement assay; triplex dna; assay detection ... See more keywords
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Space-confined indicator displacement assay inside a metal–organic framework for fluorescence turn-on sensing† †Electronic supplementary information (ESI) available: Supplementary graphics, Fig. S1–S13. See DOI: 10.1039/c9sc00032a

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Published in 2019 at "Chemical Science"

DOI: 10.1039/c9sc00032a

Abstract: Taking advantage of space-confined competing supramolecular interactions, the indicator displacement assay was performed within a recyclable MOF, which allows for fluorescence turn-on sensing with dramatically improved sensitivity and anti-interference read more here.

Keywords: indicator displacement; turn sensing; displacement assay; space confined ... See more keywords
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Tetralactam macrocycle based indicator displacement assay for colorimetric and fluorometric dual-mode detection of urinary uric acid.

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Published in 2023 at "Chemical communications"

DOI: 10.1039/d2cc06622j

Abstract: An indicator displacement assay for colorimetric and fluorometric dual-mode detection of urinary uric acid (UA) was constructed using a water-soluble naphthalene-based tetralactam macrocycle and the phenoxazine dye, resorufin (RF). The visual detection of UA levels… read more here.

Keywords: colorimetric fluorometric; displacement assay; fluorometric dual; indicator displacement ... See more keywords
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A rapid fluorescent indicator displacement assay and principal component/cluster data analysis for determination of ligand–nucleic acid structural selectivity

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Published in 2018 at "Nucleic Acids Research"

DOI: 10.1093/nar/gky019

Abstract: Abstract We describe a rapid fluorescence indicator displacement assay (R-FID) to evaluate the affinity and the selectivity of compounds binding to different DNA structures. We validated the assay using a library of 30 well-known nucleic… read more here.

Keywords: indicator displacement; displacement assay; principal component; analysis ... See more keywords
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Development of a New Colorimetric Chemosensor for Selective Determination of Urinary and Vegetable Oxalate Concentration Through an Indicator Displacement Assay (IDA) in Aqueous Media

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Published in 2018 at "Food Technology and Biotechnology"

DOI: 10.17113/ftb.56.03.18.5726

Abstract: SUMMARY The paper proposes a method that exhibits operational simplicity for the indirect spectrophotometric determination of oxalate ion. We developed Reactive Blue 4 (RB4) as a sensor by complexation with copper ion as a simple,… read more here.

Keywords: indicator displacement; oxalate concentration; displacement assay; determination ... See more keywords