Phage Display Libraries: From Binders to Targeted Drug Delivery and Human Therapeutics
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Biotechnology"
DOI: 10.1007/s12033-019-00156-8
Abstract: The application of repertoire selection technologies for the study and characterization of tumor heterogeneity is an area of great interest in the field of tumor biology and immunotherapy. Among the most promising approaches, phage display… read more here.
Keywords: human therapeutics; phage display; display libraries; delivery ... See more keywords
Enzymatic Macrolactamization of mRNA Display Libraries for Inhibitor Selection.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00828
Abstract: mRNA display is a powerful, high-throughput technology for discovering novel, peptide ligands for protein targets. A number of methods have been used to expand the chemical diversity of mRNA display libraries beyond the 20 canonical… read more here.
Keywords: display; mrna display; display libraries; cyclization ... See more keywords
Oxford nanopore sequencing enables rapid discovery of single-domain antibodies from phage display libraries.
Sign Up to like & getrecommendations! Published in 2018 at "BioTechniques"
DOI: 10.2144/btn-2018-0123
Abstract: Antibody (Ab) repertoire sequencing using high-throughput massively parallel technologies has contributed substantially to the understanding of Ab responses following infection, vaccination and autoimmunity. Because individual B-cell receptors are recombined and diversified somatically, genomic comparisons are… read more here.
Keywords: phage display; display libraries; single domain; nanopore sequencing ... See more keywords
Stability-Diversity Tradeoffs Impose Fundamental Constraints on Selection of Synthetic Human VH/VL Single-Domain Antibodies from In Vitro Display Libraries
Sign Up to like & getrecommendations! Published in 2017 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2017.01759
Abstract: Human autonomous VH/VL single-domain antibodies (sdAbs) are attractive therapeutic molecules, but often suffer from suboptimal stability, solubility and affinity for cognate antigens. Most commonly, human sdAbs have been isolated from in vitro display libraries constructed… read more here.
Keywords: vitro display; display libraries; selection; single domain ... See more keywords
Screening of Yeast Display Libraries of Enzymatically Treated Peptides to Discover Macrocyclic Peptide Ligands
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms22041634
Abstract: We present the construction and screening of yeast display libraries of post-translationally modified peptides wherein site-selective enzymatic treatment of linear peptides is achieved using bacterial transglutaminase. To this end, we developed two alternative routes, namely… read more here.
Keywords: peptide; yeast display; display libraries; treated peptides ... See more keywords