Structural prediction of protein models using distance restraints derived from cross-linking mass spectrometry data.
Sign Up to like & getrecommendations! Published in 2018 at "Nature protocols"
DOI: 10.1038/nprot.2017.146
Abstract: This protocol describes a workflow for creating structural models of proteins or protein complexes using distance restraints derived from cross-linking mass spectrometry experiments. The distance restraints are used (i) to adjust preliminary models that are… read more here.
Keywords: restraints derived; cross; using distance; cross linking ... See more keywords
Methodology for rigorous modeling of protein conformational changes by Rosetta using DEER distance restraints
Sign Up to like & getrecommendations! Published in 2021 at "PLoS Computational Biology"
DOI: 10.1371/journal.pcbi.1009107
Abstract: We describe an approach for integrating distance restraints from Double Electron-Electron Resonance (DEER) spectroscopy into Rosetta with the purpose of modeling alternative protein conformations from an initial experimental structure. Fundamental to this approach is a… read more here.
Keywords: deer; methodology; modeling protein; protein conformational ... See more keywords