Disulfide bond formation and redox regulation in the Golgi apparatus
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Letters"
DOI: 10.1002/1873-3468.14510
Abstract: Formation of disulfide bonds in secreted and cell‐surface proteins involves numerous enzymes and chaperones abundant in the endoplasmic reticulum (ER), the first and main site for disulfide bonding in the secretory pathway. Although the Golgi… read more here.
Keywords: disulfide; formation; golgi apparatus; golgi ... See more keywords
Improving the thermal stability of azoreductase from Halomonas elongata by introducing a disulfide bond via site‐directed mutagenesis
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.1688
Abstract: Azoreductases mainly reduce azo dyes, the largest class of colorants, to colorless aromatic amines. AzoH, a new azoreductase from the halophilic bacterium, Halomonas elongata, has been recently cloned and expressed in Escherichia coli. The aim… read more here.
Keywords: disulfide bond; azoreductase; halomonas elongata; stability ... See more keywords
Impact of depth filtration on disulfide bond reduction during downstream processing of monoclonal antibodies from CHO cell cultures
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26964
Abstract: Monoclonal antibody interchain disulfide bond reduction was observed in a Chinese Hamster Ovary manufacturing process that used single‐use technologies. A similar reduction has been reported for processes that involved high mechanical shear recovery unit operations,… read more here.
Keywords: reduction; disulfide bond; depth filtration; bond reduction ... See more keywords
2,2′‐Dipyridyl diselenide: A chemoselective tool for cysteine deprotection and disulfide bond formation
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Peptide Science"
DOI: 10.1002/psc.3236
Abstract: There are many examples of bioactive, disulfide‐rich peptides and proteins whose biological activity relies on proper disulfide connectivity. Regioselective disulfide bond formation is a strategy for the synthesis of these bioactive peptides, but many of… read more here.
Keywords: cys; disulfide bond; bond formation; formation ... See more keywords
Aqueous ionic liquids influence the disulfide bond isoform equilibrium in conotoxin AuIB: a consequence of the Hofmeister effect?
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Reviews"
DOI: 10.1007/s12551-017-0391-2
Abstract: The appearance of several disulfide bond isoforms in multiple cysteine containing venom peptides poses a significant challenge in their synthesis and purification under laboratory conditions. Recent experiments suggest that careful tuning of solvent and temperature… read more here.
Keywords: disulfide bond; isoform equilibrium; bond; aqueous ionic ... See more keywords
Cysteine mediated disulfide bond formation in RAGE V domain facilitates its functionally relevant dimerization.
Sign Up to like & getrecommendations! Published in 2018 at "Biochimie"
DOI: 10.1016/j.biochi.2018.07.024
Abstract: Receptor for Advanced Glycation End product (RAGE) is a multiligand receptor implicated in diverse pathological conditions such as diabetes, atherosclerosis, cancer and neural diseases. Extracellular, RAGE consists of V, C1 and C2 domains. Here, we… read more here.
Keywords: disulfide bond; mediated disulfide; bond formation; rage ... See more keywords
Improved production of single domain antibodies with two disulfide bonds by co-expression of chaperone proteins in the Escherichia coli periplasm.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of immunological methods"
DOI: 10.1016/j.jim.2017.01.007
Abstract: Single domain antibodies are recombinantly expressed variable domains derived from camelid heavy chain antibodies. Natural single domain antibodies can have noncanonical disulfide bonds between their complementarity-determining regions that help position the binding site. In addition,… read more here.
Keywords: domain antibodies; disulfide bond; disulfide bonds; single domain ... See more keywords
Disulfide bond characterization of human factor Xa by mass spectrometry through protein‐level partial reduction
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Pharmaceutical and Biomedical Analysis"
DOI: 10.1016/j.jpba.2016.10.005
Abstract: HighlightsProtein‐level partial reduction is proposed first time in complex disulfide linkage study.Disulfide bonds in Human Factor Xa are fully characterized first time by MS‐based method.Partial reduction can be used for disulfide bond mapping in recombinant… read more here.
Keywords: reduction; disulfide bond; human factor; partial reduction ... See more keywords
Dmab/ivDde protected diaminodiacids for solid-phase synthesis of peptide disulfide-bond mimics
Sign Up to like & getrecommendations! Published in 2017 at "Tetrahedron Letters"
DOI: 10.1016/j.tetlet.2017.03.024
Abstract: The use of pre-prepared diaminodiacids has been established as an effective approach for the chemical synthesis of peptide disulfide bond mimics. A technical problem often encountered in the implementation of the diaminodiacids strategy is the… read more here.
Keywords: peptide disulfide; disulfide bond; synthesis peptide; bond mimics ... See more keywords
IgE Recognition and Structural Analysis of Disulfide Bond Rearrangement and Chemical Modifications in Allergen Aggregations in Roasted Peanuts.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.3c01482
Abstract: Given that roasting changes the structure and allergenicity of peanut allergens, the structural information of peanut allergens must be expounded to explain the alteration in their allergenicity. This work focused on allergen aggregations (AAs) in… read more here.
Keywords: allergen aggregations; roasted peanuts; bond rearrangement; ige recognition ... See more keywords
1,2,3-Triazole Rings as a Disulfide Bond Mimetic in Chimeric AGRP-Melanocortin Peptides: Design, Synthesis, and Functional Characterization.
Sign Up to like & getrecommendations! Published in 2018 at "ACS chemical neuroscience"
DOI: 10.1021/acschemneuro.7b00422
Abstract: The melanocortin system is involved in the regulation of complex physiological functions, including energy and weight homeostasis, feeding behavior, inflammation, sexual function, pigmentation, and exocrine gland function. The five melanocortin receptors that belong to the… read more here.
Keywords: disulfide bond; melanocortin receptors; agrp melanocortin; chimeric agrp ... See more keywords