Improving thermostability of (R)‐selective amine transaminase from Aspergillus terreus through introduction of disulfide bonds
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.1572
Abstract: To improve the thermostability of (R)‐selective amine transaminase from Aspergillus terreus (AT‐ATA), we used computer software Disulfide by Design and Modelling of Disulfide Bonds in Proteins to identify mutation sites where the disulfide bonds were… read more here.
Keywords: selective amine; disulfide bonds; thermostability; amine transaminase ... See more keywords
Bridges to Stability: Engineering Disulfide Bonds Towards Enhanced Lipase Biodiesel Synthesis
Sign Up to like & getrecommendations! Published in 2019 at "ChemCatChem"
DOI: 10.1002/cctc.201901369
Abstract: Computational design of disulfide bonds was performed for lipase from Geobacillus stearothermophilus T6 (LipT6) for enhanced methanol stability and improved biodiesel production. Thirteen double mutants comprising new cysteine pairs were screened and evaluated for their… read more here.
Keywords: stability; biodiesel synthesis; disulfide bonds;
Impact of disulfide bonds on the folding and refolding capability of a novel thermostable GH45 cellulase
Sign Up to like & getrecommendations! Published in 2018 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-018-9256-2
Abstract: A new cellulase (TaCel45) of glycoside hydrolase family 45 was identified in the thermophilic fungus Thielavia arenaria XZ7 and was successfully expressed in Pichia pastoris. The specific activities of TaCel45 towards lichenin, sodium carboxymethylcellulose (CMC-Na),… read more here.
Keywords: gh45 cellulase; disulfide bonds; refolding capability; folding refolding ... See more keywords
Role of Disulfide Bonds on DNA Packaging Forces in Bull Sperm Chromatin.
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical journal"
DOI: 10.1016/j.bpj.2017.08.050
Abstract: Short arginine-rich proteins called protamines mediate the near crystalline DNA packaging in most vertebrate sperm cells. Protamines are synthesized during spermiogenesis and condense the paternal genome into a transcriptionally inactive state in late-stage spermatids. Protamines… read more here.
Keywords: disulfide bonds; dna packaging; bonds dna; role disulfide ... See more keywords
Improved production of single domain antibodies with two disulfide bonds by co-expression of chaperone proteins in the Escherichia coli periplasm.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of immunological methods"
DOI: 10.1016/j.jim.2017.01.007
Abstract: Single domain antibodies are recombinantly expressed variable domains derived from camelid heavy chain antibodies. Natural single domain antibodies can have noncanonical disulfide bonds between their complementarity-determining regions that help position the binding site. In addition,… read more here.
Keywords: domain antibodies; disulfide bond; disulfide bonds; single domain ... See more keywords
Integrin αvβ3 and disulfide bonds play important roles in NGR-retargeted adenovirus transduction efficiency.
Sign Up to like & getrecommendations! Published in 2021 at "Life sciences"
DOI: 10.1016/j.lfs.2021.120116
Abstract: AIMS Adenoviruses that have CNGRCVSGCAGRC peptide inserted into fiber (AdFNGR) or hexon (AdHNGR) protein, respectively, showed increased transduction of endothelial cells. In this study we investigated if cysteines within the CNGRCVSGCAGRC sequence inserted into Ad5… read more here.
Keywords: disulfide bonds; transduction efficiency; adfngr adhngr; ngr retargeted ... See more keywords
Improvement of multiple-responsive shape memory effects of wool through increasing the content of disulfide bonds
Sign Up to like & getrecommendations! Published in 2020 at "Polymer"
DOI: 10.1016/j.polymer.2019.122130
Abstract: Abstract As an ideal smart wearable natural material, α-keratin type mammalian hairs have proved to exhibit multiple-responsive shape memory effects under water, wet, xenon and redox stimuli, in which the inherent disulfide bonds played the… read more here.
Keywords: disulfide bonds; shape; shape memory; multiple responsive ... See more keywords
Reprogrammable, Reprocessible, and Self-Healable Liquid Crystal Elastomer with Exchangeable Disulfide Bonds.
Sign Up to like & getrecommendations! Published in 2017 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.7b09246
Abstract: A liquid crystal elastomer (LCE) can be regarded as an integration of mesogenic molecules into a polymer network. The LCE can generate large mechanical actuation when subjected to various external stimuli. Recently, it has been… read more here.
Keywords: crystal elastomer; liquid crystal; disulfide bonds; lce ... See more keywords
Reactive Epoxy Nanofiltration Membranes with Disulfide Bonds for the Separation of Multicomponent Chemical Mixtures
Sign Up to like & getrecommendations! Published in 2018 at "ACS Omega"
DOI: 10.1021/acsomega.8b00931
Abstract: This article reports the fabrication of organic solvent nanofiltration membranes containing a labile disulfide bond, which is broken by reaction with a chemical stimulus. These membranes are a new generation of smart membranes that have… read more here.
Keywords: disulfide bonds; fraction contained; separation; chemical ... See more keywords
Stereodivergent Chirality Transfer by Noncovalent Control of Disulfide Bonds
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.1c10000
Abstract: Controlling dynamic stereochemistry is an important challenge, as it is not only inherent to protein structure and function but often governs supramolecular systems and self-assembly. Typically, disulfide bonds exhibit stereodivergent behavior in proteins; however, how… read more here.
Keywords: stereodivergent chirality; chirality transfer; disulfide bonds; control disulfide ... See more keywords
Investigation of Product Ions Generated by 193 nm Ultraviolet Photodissociation of Peptides and Proteins Containing Disulfide Bonds.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.2c00124
Abstract: Disulfide bridges are unique post-translational modifications (PTM) that contribute to protein architecture and modulate function. This PTM, however, challenges top-down mass spectrometry by cyclizing stretches of the protein sequence. In order to produce and release… read more here.
Keywords: disulfide bonds; ultraviolet photodissociation; 193 ultraviolet; product ions ... See more keywords