Discovery of Diverse CRISPR-Cas Systems and Expansion of the Genome Engineering Toolbox.
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DOI: 10.1021/acs.biochem.3c00159
Abstract: CRISPR systems mediate adaptive immunity in bacteria and archaea through diverse effector mechanisms and have been repurposed for versatile applications in therapeutics and diagnostics thanks to their facile reprogramming with RNA guides. RNA-guided CRISPR-Cas targeting… read more here.
Keywords: crispr; diverse crispr; class; effector ... See more keywords
A catalogue of biochemically diverse CRISPR-Cas9 orthologs
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DOI: 10.1038/s41467-020-19344-1
Abstract: Bacterial Cas9 nucleases from type II CRISPR-Cas antiviral defence systems have been repurposed as genome editing tools. Although these proteins are found in many microbes, only a handful of variants are used for these applications.… read more here.
Keywords: crispr; cas9 orthologs; biochemically diverse; diverse crispr ... See more keywords