A non-G-Quadruplex DNA Aptamer Targeting NCL for Diagnosis and Therapy in Bladder Cancer.
Sign Up to like & getrecommendations! Published in 2023 at "Advanced healthcare materials"
DOI: 10.1002/adhm.202300791
Abstract: Bladder cancer (BC) is a highly aggressive malignant tumor affecting the urinary system, characterized by metastasis and a poor prognosis that often leads to limited therapeutic success. In this study, we aimed to develop a… read more here.
Keywords: dna aptamer; non quadruplex; bladder cancer; cancer ... See more keywords
Isolation of a novel DNA aptamer against LipL32 as a potential diagnostic agent for the detection of pathogenic Leptospira
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202200418
Abstract: Leptospirosis is a potentially life‐threatening zoonosis caused by pathogenic Leptospira and for rapid diagnostics, direct detection is desirable. LipL32 protein is the most suitable biomarker for direct detection. DNA aptamers are sought to be generated… read more here.
Keywords: dna aptamer; pathogenic leptospira; detection; aptamer ... See more keywords
In Vitro Selection of a DNA Aptamer by Cell-SELEX as a Molecular Probe for Cervical Cancer Recognition and Imaging
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Molecular Evolution"
DOI: 10.1007/s00239-019-9886-8
Abstract: Aptamers have become the most promising recognition reagents in terms of early diagnosis and effective treatment of cancers. In this study, using cervical cancer as a model, we have identified a DNA aptamer specifically binding… read more here.
Keywords: cervical cancer; cell selex; recognition; cancer ... See more keywords
Construction of DNA-NanoLuc luciferase conjugates for DNA aptamer-based sandwich assay using Rep protein
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Letters"
DOI: 10.1007/s10529-018-02641-7
Abstract: ObjectiveWe developed a DNA-NanoLuc luciferase (NnaoLuc) conjugates for DNA aptamer-based sandwich assay using the catalytic domain of the replication initiator protein derived from porcine circovirus type 2 (pRep).ResultsFor construction of DNA aptamer and NanoLuc conjugate… read more here.
Keywords: sandwich assay; based sandwich; aptamer based; dna nanoluc ... See more keywords
Gold nanoparticle-DNA aptamer-assisted delivery of antimicrobial peptide effectively inhibits Acinetobacter baumannii infection in mice
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Microbiology"
DOI: 10.1007/s12275-022-1620-3
Abstract: Acinetobacter baumannii causes multidrug resistance, leading to fatal infections in humans. In this study, we showed that Lys AB2 P3-His-a hexahistidine-tagged form of an antimicrobial peptide (AMP) loaded onto DNA aptamer-functionalized gold nanoparticles (AuNP-Apt)-can effectively… read more here.
Keywords: aunp apt; antimicrobial peptide; acinetobacter baumannii; mice ... See more keywords
Developing a DNA aptamer-based approach for biosensing cystatin-c in serum: An alternative to antibody-based methods.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2019.113386
Abstract: Oligonucleotide aptamers are short, synthetic and single-stranded DNA or RNA molecules capable of binding to a wide range of molecules, from small molecules to large cells. Nowadays, aptamers are valuable tools in research, clinical diagnosis… read more here.
Keywords: dna; aptamer based; approach biosensing; based approach ... See more keywords
Electrochemical detection of β-lactoglobulin based on a highly selective DNA aptamer and flower-like Au@BiVO4 microspheres.
Sign Up to like & getrecommendations! Published in 2020 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2020.04.066
Abstract: Beta-lactoglobulin is a natural milk protein and the main cause of infant milk allergy. In this work, a sensitive, selective and inexpensive electrochemical biosensor for the detection of β-lactoglobulin was developed. In this sensor, a… read more here.
Keywords: dna aptamer; detection; bivo4; detection lactoglobulin ... See more keywords
DNA aptamer immobilized hydroxyapatite for enhancing angiogenesis and bone regeneration.
Sign Up to like & getrecommendations! Published in 2019 at "Acta biomaterialia"
DOI: 10.1016/j.actbio.2019.08.047
Abstract: In this study, we developed aptamer-conjugated hydroxyapatite (Apt-HA) that promotes bone regeneration and angiogenesis. The 3R02 bivalent aptamer specific to vascular endothelial growth factor (VEGF) was grafted to the hydroxyapatite (HA) surface. Apt-HA was tested… read more here.
Keywords: bone; dna aptamer; angiogenesis bone; bone regeneration ... See more keywords
DNA aptamer probes for detection of estrogen receptor &agr; positive carcinomas
Sign Up to like & getrecommendations! Published in 2017 at "Translational Research"
DOI: 10.1016/j.trsl.2016.12.008
Abstract: &NA; Estrogen receptor alpha (ER&agr;) also known as NR3A1 (nuclear receptor subfamily 3, group A, member 1) is a ligand‐activated transcription factor. It is an important biomarker for breast cancer metastasis. In the present study,… read more here.
Keywords: receptor; dna aptamer; estrogen receptor; agr positive ... See more keywords
Primary Amine-Clustered DNA Aptamer for DNA-Protein Conjugation Catalyzed by Microbial Transglutaminase.
Sign Up to like & getrecommendations! Published in 2017 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.7b00594
Abstract: DNA-protein conjugates are promising biomolecules for use in areas ranging from therapeutics to analysis because of the dual functionalities of DNA and protein. Conjugation requires site-specific and efficient covalent bond formation without impairing the activity… read more here.
Keywords: dna aptamer; primary amine; dna protein; dna ... See more keywords
Detection of SARS-CoV-2 RNA Using a DNA Aptamer Mimic of Green Fluorescent Protein.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.1c00893
Abstract: RNA detection is important in diverse diagnostic and analytical applications. RNAs can be rapidly detected using molecular beacons, which fluoresce upon hybridizing to a target RNA but require oligonucleotides with complex fluorescent dye and quencher… read more here.
Keywords: dna aptamer; rna; detection; rna using ... See more keywords