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   Articles with "dna assembly" as a keyword



High fidelity one‐pot DNA assembly using orthogonal serine integrases

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recommendations! Published in 2022 at "Biotechnology Journal"

DOI: 10.1002/biot.202200411

Abstract: Large serine integrases (LSIs, derived from temperate phages) have been adapted for use in a multipart DNA assembly process in vitro, called serine integrase recombinational assembly (SIRA). The versatility, efficiency, and fidelity of SIRA is… read more here.

Keywords: serine integrases; dna assembly; high fidelity;

BASIC: A Simple and Accurate Modular DNA Assembly Method.

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recommendations! Published in 2017 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-6343-0_6

Abstract: Biopart Assembly Standard for Idempotent Cloning (BASIC) is a simple, accurate, and robust DNA assembly method. The method is based on linker-mediated DNA assembly and provides highly accurate DNA assembly with 99 % correct assemblies… read more here.

Keywords: simple accurate; assembly method; dna assembly; assembly ... See more keywords

Multipart DNA Assembly Using Site-Specific Recombinases from the Large Serine Integrase Family.

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recommendations! Published in 2017 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-7169-5_19

Abstract: Assembling multiple DNA fragments into functional plasmids is an important and often rate-limiting step in engineering new functions in living systems. Bacteriophage integrases are enzymes that carry out efficient recombination reactions between short, defined DNA sequences… read more here.

Keywords: integrase; assembly using; dna; serine integrase ... See more keywords

Surface Functionalization and Reversible Disassembly of DNA-Assembly Nanoparticles for Sensitive and Multiplexed Detection of DNA Targets Without Enzymatic and Catalytic Amplification.

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recommendations! Published in 2023 at "Bioconjugate chemistry"

DOI: 10.1021/acs.bioconjchem.3c00143

Abstract: Recently, DNA-assembly nanoparticles based on DNA-metal ion interactions are emerging as new building blocks for drug delivery and metal nanostructure synthesis. However, the surface modification of DNA-assembly nanoparticles using functional biomolecules that can identify specific… read more here.

Keywords: assembly nanoparticles; dna; bcdna; detection ... See more keywords

Spatioselective Imaging of Noncoding RNAs in Mitochondria via an Organelle-Specific DNA Assembly Strategy.

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recommendations! Published in 2025 at "Nano letters"

DOI: 10.1021/acs.nanolett.4c05559

Abstract: Precise imaging of noncoding RNAs (ncRNAs) in specific organelles allows decoding of their functions at subcellular level but lacks advanced tools. Here we present a DNA-based nanobiotechnology for spatially selective imaging of ncRNA (e.g., microRNA… read more here.

Keywords: imaging noncoding; dna assembly; noncoding rnas; strategy ... See more keywords

DNA Assembly of Modular Components into a Rotary Nanodevice.

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recommendations! Published in 2022 at "ACS nano"

DOI: 10.1021/acsnano.1c10160

Abstract: The bacterial flagellar motor is a rotary machine composed of functional modular components, which can perform bidirectional rotations to control the migration behavior of the bacterial cell. It resembles a two-cogwheel gear system, which consists… read more here.

Keywords: dna assembly; assembly modular; components rotary; rotary nanodevice ... See more keywords

Phage Enzyme-Assisted Direct In Vivo DNA Assembly in Multiple Microorganisms.

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recommendations! Published in 2022 at "ACS synthetic biology"

DOI: 10.1021/acssynbio.1c00529

Abstract: The assembly of DNA fragments is extremely important for molecular biology. Increasing numbers of studies have focused on streamlining the laborious and costly protocols via in vivo DNA assembly. However, the existing methods were mainly… read more here.

Keywords: vivo dna; dna; direct vivo; dna assembly ... See more keywords

In Vivo DNA Assembly in Yarrowia lipolytica

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recommendations! Published in 2025 at "ACS Synthetic Biology"

DOI: 10.1021/acssynbio.5c00296

Abstract: The oleaginous yeast Yarrowia lipolytica is an important platform organism for biotechnology applications. In this study, we established an in vivo DNA assembly system leveraging CRISPR-Cas9 for efficient genomic integration of multiple DNA fragments into… read more here.

Keywords: dna assembly; yarrowia lipolytica; vivo dna; assembly yarrowia ... See more keywords

Nongenetic Approach for Imaging Protein Dimerization by Aptamer Recognition and Proximity-Induced DNA Assembly.

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recommendations! Published in 2018 at "Journal of the American Chemical Society"

DOI: 10.1021/jacs.7b11311

Abstract: Herein, we report a nongenetic and real-time approach for imaging protein dimerization on living cell surfaces by aptamer recognition and proximity-induced DNA assembly. We use the aptamer specific for the receptor monomer as a recognition… read more here.

Keywords: dimerization; dna assembly; recognition; approach ... See more keywords

basicsynbio and the BASIC SEVA collection: software and vectors for an established DNA assembly method

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recommendations! Published in 2022 at "Synthetic Biology"

DOI: 10.1093/synbio/ysac023

Abstract: Standardized DNA assembly methods utilizing modular components provide a powerful framework to explore design spaces and iterate through Design-Build-Test-Learn cycles. Biopart Assembly Standard for Idempotent Cloning (BASIC) DNA assembly uses modular parts and linkers, is… read more here.

Keywords: seva; collection; dna assembly; basicsynbio ... See more keywords

Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly

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recommendations! Published in 2018 at "PLoS ONE"

DOI: 10.1371/journal.pone.0189892

Abstract: Synthetic biology builds upon the foundation of engineering principles, prompting innovation and improvement in biotechnology via a design-build-test-learn cycle. A community-wide standard in DNA assembly would enable bio-molecular engineering at the levels of predictivity and… read more here.

Keywords: mobius assembly; assembly; golden gate; dna assembly ... See more keywords