High fidelity one‐pot DNA assembly using orthogonal serine integrases
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202200411
Abstract: Large serine integrases (LSIs, derived from temperate phages) have been adapted for use in a multipart DNA assembly process in vitro, called serine integrase recombinational assembly (SIRA). The versatility, efficiency, and fidelity of SIRA is… read more here.
Keywords: serine integrases; dna assembly; high fidelity;
BASIC: A Simple and Accurate Modular DNA Assembly Method.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6343-0_6
Abstract: Biopart Assembly Standard for Idempotent Cloning (BASIC) is a simple, accurate, and robust DNA assembly method. The method is based on linker-mediated DNA assembly and provides highly accurate DNA assembly with 99 % correct assemblies… read more here.
Keywords: simple accurate; assembly method; dna assembly; assembly ... See more keywords
Multipart DNA Assembly Using Site-Specific Recombinases from the Large Serine Integrase Family.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7169-5_19
Abstract: Assembling multiple DNA fragments into functional plasmids is an important and often rate-limiting step in engineering new functions in living systems. Bacteriophage integrases are enzymes that carry out efficient recombination reactions between short, defined DNA sequences… read more here.
Keywords: integrase; assembly using; dna; serine integrase ... See more keywords
Surface Functionalization and Reversible Disassembly of DNA-Assembly Nanoparticles for Sensitive and Multiplexed Detection of DNA Targets Without Enzymatic and Catalytic Amplification.
Sign Up to like & getrecommendations! Published in 2023 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.3c00143
Abstract: Recently, DNA-assembly nanoparticles based on DNA-metal ion interactions are emerging as new building blocks for drug delivery and metal nanostructure synthesis. However, the surface modification of DNA-assembly nanoparticles using functional biomolecules that can identify specific… read more here.
Keywords: assembly nanoparticles; dna; bcdna; detection ... See more keywords
DNA Assembly of Modular Components into a Rotary Nanodevice.
Sign Up to like & getrecommendations! Published in 2022 at "ACS nano"
DOI: 10.1021/acsnano.1c10160
Abstract: The bacterial flagellar motor is a rotary machine composed of functional modular components, which can perform bidirectional rotations to control the migration behavior of the bacterial cell. It resembles a two-cogwheel gear system, which consists… read more here.
Keywords: dna assembly; assembly modular; components rotary; rotary nanodevice ... See more keywords
Phage Enzyme-Assisted Direct In Vivo DNA Assembly in Multiple Microorganisms.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00529
Abstract: The assembly of DNA fragments is extremely important for molecular biology. Increasing numbers of studies have focused on streamlining the laborious and costly protocols via in vivo DNA assembly. However, the existing methods were mainly… read more here.
Keywords: vivo dna; dna; direct vivo; dna assembly ... See more keywords
Nongenetic Approach for Imaging Protein Dimerization by Aptamer Recognition and Proximity-Induced DNA Assembly.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.7b11311
Abstract: Herein, we report a nongenetic and real-time approach for imaging protein dimerization on living cell surfaces by aptamer recognition and proximity-induced DNA assembly. We use the aptamer specific for the receptor monomer as a recognition… read more here.
Keywords: dimerization; dna assembly; recognition; approach ... See more keywords
basicsynbio and the BASIC SEVA collection: software and vectors for an established DNA assembly method
Sign Up to like & getrecommendations! Published in 2022 at "Synthetic Biology"
DOI: 10.1093/synbio/ysac023
Abstract: Standardized DNA assembly methods utilizing modular components provide a powerful framework to explore design spaces and iterate through Design-Build-Test-Learn cycles. Biopart Assembly Standard for Idempotent Cloning (BASIC) DNA assembly uses modular parts and linkers, is… read more here.
Keywords: seva; collection; dna assembly; basicsynbio ... See more keywords
Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly
Sign Up to like & getrecommendations! Published in 2018 at "PLoS ONE"
DOI: 10.1371/journal.pone.0189892
Abstract: Synthetic biology builds upon the foundation of engineering principles, prompting innovation and improvement in biotechnology via a design-build-test-learn cycle. A community-wide standard in DNA assembly would enable bio-molecular engineering at the levels of predictivity and… read more here.
Keywords: mobius assembly; assembly; golden gate; dna assembly ... See more keywords
HyperXpress: Rapid Single Vessel DNA Assembly and Protein Production in Microliterscale
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.832176
Abstract: Rapid prototyping of biological functions has the common aim of generating, screening, and selecting variant libraries as quickly as possible. This approach is now to be extended by the HyperXpress workflow, which connects ligase cycling… read more here.
Keywords: single vessel; dna assembly; hyperxpress; protein ... See more keywords