Comet Assay for the Detection of Single and Double-Strand DNA Breaks.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1948-3_18
Abstract: DNA damage is a common event that occurs during the life span of the majority of cells, as a result of aberrant energy metabolism, exposure to radiation, DNA damaging chemicals, and viral infections. Rapid, sensitive,… read more here.
Keywords: dna; single double; double stranded; comet assay ... See more keywords
Bridging of DNA breaks activates PARP2–HPF1 to modify chromatin
Sign Up to like & getrecommendations! Published in 2020 at "Nature"
DOI: 10.1038/s41586-020-2725-7
Abstract: Breaks in DNA strands recruit the protein PARP1 and its paralogue PARP2 to modify histones and other substrates through the addition of mono- and poly(ADP-ribose) (PAR) 1 – 5 . In the DNA damage responses,… read more here.
Keywords: parp2 hpf1; dna breaks; chromatin; dna ... See more keywords
AZD1775 induces toxicity through double-stranded DNA breaks independently of chemotherapeutic agents in p53-mutated colorectal cancer cells
Sign Up to like & getrecommendations! Published in 2017 at "Cell Cycle"
DOI: 10.1080/15384101.2017.1301329
Abstract: ABSTRACT AZD1775 is a small molecule WEE1 inhibitor used in combination with DNA-damaging agents to cause premature mitosis and cell death in p53-mutated cancer cells. Here we sought to determine the mechanism of action of… read more here.
Keywords: dna breaks; cancer cells; p53 mutated; dna ... See more keywords
Producing irreversible topoisomerase II-mediated DNA breaks by site-specific Pt(II)-methionine coordination chemistry
Sign Up to like & getrecommendations! Published in 2017 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkx742
Abstract: Abstract Human type II topoisomerase (Top2) isoforms, hTop2α and hTop2β, are targeted by some of the most successful anticancer drugs. These drugs induce Top2-mediated DNA cleavage to trigger cell-death pathways. The potency of these drugs… read more here.
Keywords: chemistry; dna breaks; drug; topoisomerase ... See more keywords
DNA fragmentation of human spermatozoa: Simple assessment of single‐ and double‐strand DNA breaks and their respective dynamic behavioral response
Sign Up to like & getrecommendations! Published in 2020 at "Andrology"
DOI: 10.1111/andr.12819
Abstract: Procedures to detect sperm DNA fragmentation (SDF), like the sperm chromatin dispersion (SCD) test, determine the “global” SDF without discriminating between spermatozoa with single‐strand DNA breaks only (SDF‐SSBs) and those containing double‐strand DNA breaks (SDF‐DSBs). read more here.
Keywords: dna; strand dna; dna breaks; double strand ... See more keywords
Cancer cells use self-inflicted DNA breaks to evade growth limits imposed by genotoxic stress
Sign Up to like & getrecommendations! Published in 2022 at "Science"
DOI: 10.1126/science.abi6378
Abstract: Genotoxic therapy such as radiation serves as a frontline cancer treatment, yet acquired resistance that leads to tumor reoccurrence is frequent. We found that cancer cells maintain viability during irradiation by reversibly increasing genome-wide DNA… read more here.
Keywords: dna; dna breaks; self inflicted; tumor ... See more keywords
Repair of DNA Breaks by Break-Induced Replication.
Sign Up to like & getrecommendations! Published in 2021 at "Annual review of biochemistry"
DOI: 10.1146/annurev-biochem-081420-095551
Abstract: Double-strand DNA breaks (DSBs) are the most lethal type of DNA damage, making DSB repair critical for cell survival. However, some DSB repair pathways are mutagenic and promote genome rearrangements, leading to genome destabilization. One… read more here.
Keywords: biochemistry; dna breaks; break induced; repair ... See more keywords
Meiotic DNA breaks activate a streamlined phospho-signaling response that largely avoids protein-level changes
Sign Up to like & getrecommendations! Published in 2022 at "Life Science Alliance"
DOI: 10.26508/lsa.202201454
Abstract: The meiotic DNA break response is shown to involve a large number of phosphorylation changes and a clearly detectable transcriptional response. By contrast, the meiotic proteome remains remarkably unchanged in response to breaks. Meiotic cells… read more here.
Keywords: dna; meiotic dna; dna breaks; dna break ... See more keywords
Let’s not take DNA breaks for granted. The importance of direct detection of DNA breaks for the successful development of DDR inhibitors
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2023.1118716
Abstract: Successful development of a drug candidate requires availability of robust methods that enable precise and quantitative assessment of the biological effects exerted by the molecule of interest. In case of DNA Damage Response inhibitors, the… read more here.
Keywords: successful development; dna; dna damage; dna breaks ... See more keywords