Modular Assembly of a Concatenated DNA Circuit for In Vivo Amplified Aptasensing.
Sign Up to like & getrecommendations! Published in 2022 at "Small"
DOI: 10.1002/smll.202200983
Abstract: Probing endogenous molecular profiles in living entities is of fundamental significance to decipher biological functions and exploit novel theranostics. Despite programmable nucleic acid-based aptasensing systems across the breadth of molecular imaging, an aptasensing system enabling… read more here.
Keywords: concatenated dna; dna; assembly concatenated; circuit ... See more keywords
An amplifying DNA circuit coupled with Mg2+-dependent DNAzyme for bisphenol A detection in milk samples.
Sign Up to like & getrecommendations! Published in 2021 at "Food chemistry"
DOI: 10.1016/j.foodchem.2020.128975
Abstract: As bisphenol A (BPA) is harmful to human health, it is of great significance to develop a new method for BPA detection. Herein, we designed a BPA biosensor by integrating an amplifying DNA circuit with… read more here.
Keywords: detection; dna circuit; mg2 dependent; dna ... See more keywords
Dual catalytic DNA circuit-induced gold nanoparticle aggregation: An enzyme-free and colorimetric strategy for amplified detection of nucleic acids.
Sign Up to like & getrecommendations! Published in 2020 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2020.03.059
Abstract: An enzyme-free dual catalytic DNA circuit for amplified detection of nucleic acids has been developed. The system functions based on a cyclic self-assembly of two auxiliary hairpins (H1 and H2) and three biotinylated hairpin oligonucleotides… read more here.
Keywords: enzyme free; dna circuit; catalytic dna; dual catalytic ... See more keywords
Comparative Study of DNA Circuit System-Based Proportional and Exponential Amplification Strategies for Enzyme-Free and Rapid Detection of miRNA at Room Temperature
Sign Up to like & getrecommendations! Published in 2018 at "ACS Omega"
DOI: 10.1021/acsomega.7b01866
Abstract: Because circulating microRNAs (miRNAs) have been recognized as a new class of blood-based biomarkers for various diseases, a significant challenge has been the development of point-of-care testing (POCT) systems based on detection of circulating miRNAs… read more here.
Keywords: circuit system; dna circuit; amplification; dna ... See more keywords
Highly Sensitive Assay of Methyltransferase Activity Based on an Autonomous Concatenated DNA Circuit.
Sign Up to like & getrecommendations! Published in 2018 at "ACS sensors"
DOI: 10.1021/acssensors.8b00738
Abstract: Methyltransferase-involved DNA methylation is one of the most important epigenetic processes, making the ultrasensitive MTase assay highly desirable in clinical diagnosis as well as biomedical research. Traditional single-stage amplification means often achieve linear amplification that… read more here.
Keywords: cha hcr; circuit; mtase; dna circuit ... See more keywords
Nicking-Assisted Reactant Recycle to Implement Entropy-Driven DNA Circuit.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.9b07521
Abstract: Synthetic catalytic DNA circuits are important signal amplification tools for molecular programming due to their robust and modular properties. In catalytic circuits, the reactant recycling operation is essential to facilitate continuous processes. Therefore, it is… read more here.
Keywords: driven dna; entropy driven; dna circuit; dna ... See more keywords
Integrating CRISPR-Cas12a with a DNA circuit as a generic sensing platform for amplified detection of microRNA††Electronic supplementary information (ESI) available. See DOI: 10.1039/d0sc03084h
Sign Up to like & getrecommendations! Published in 2020 at "Chemical Science"
DOI: 10.1039/d0sc03084h
Abstract: A generic sensing strategy that integrates CRISPR-Cas12a with a DNA circuit is proposed for amplified detection of microRNA. read more here.
Keywords: crispr cas12a; detection microrna; dna circuit; cas12a dna ... See more keywords
Intracellular enzyme-powered DNA circuit with a tunable amplifier for miRNA imaging.
Sign Up to like & getrecommendations! Published in 2021 at "Chemical communications"
DOI: 10.1039/d1cc00536g
Abstract: We describe an intracellular enzyme-powered DNA circuit probe with a tunable amplifier for sensitive and selective detection of miRNA. This approach has been successfully applied for in situ miRNA-21 fluorescence imaging in live cells. Also,… read more here.
Keywords: dna circuit; intracellular enzyme; enzyme powered; powered dna ... See more keywords
Dynamically elongated associative toehold for tuning DNA circuit kinetics and thermodynamics
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab212
Abstract: Abstract Associative toehold is a powerful concept enabling efficient combinatorial computation in DNA circuit. A longer association length boosts circuit kinetics and equilibrium signal but results in higher leak rate. We reconcile this trade-off by… read more here.
Keywords: dna circuit; thermodynamics; associative toehold; toehold ... See more keywords