The linker region of LINEs modulates DNA cleavage and DNA polymerization.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2020.113809
Abstract: Long interspersed elements (LINEs) replicate by target primed reverse transcription (TPRT). Insertion involves two half reactions. Each half reaction involves DNA cleavage followed by DNA synthesis. The linker region, located just beyond the reverse transcriptase… read more here.
Keywords: dna cleavage; linker region; linker; dna ... See more keywords
Does Cas9-Catalyzed DNA Cleavage Generate Blunt Ends or Staggered Ends? Insight from Molecular Dynamic Simulations
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.300
Abstract: The CRISPR-associated endonuclease Cas9 from Streptococcus pyogenes (spCas9) along with a single guide RNA (sgRNA) has merged as a versatile toolbox for genome editing. Despite recent advances in the mechanistic studies on spCas9-sgRNA-mediated target double-stranded… read more here.
Keywords: dna; cleavage; staggered ends; blunt ends ... See more keywords
RNA-Independent DNA Cleavage Activities of Cas9 and Cas12a
Sign Up to like & getrecommendations! Published in 2017 at "Cell reports"
DOI: 10.1016/j.celrep.2017.11.100
Abstract: SUMMARY CRISPR-Cas systems provide bacteria and archaea with sequence-specific protection against invading mobile genetic elements. In the presence of divalent metal ions, Cas9 and Cas12a (formerly Cpf1) proteins target and cleave DNA that is complementary… read more here.
Keywords: independent dna; rna independent; cas9 cas12a; dna cleavage ... See more keywords
Microwave assisted synthesis of coumarin-purine derivatives: An approach to in vitro anti-oxidant, DNA cleavage, crystal structure, DFT studies and Hirshfeld surface analysis
Sign Up to like & getrecommendations! Published in 2019 at "Heliyon"
DOI: 10.1016/j.heliyon.2019.e01131
Abstract: An easy and efficient microwave-assisted protocol has been developed for the synthesis of coumarin-purine hybrids (3a-3j). The newly constructed 1,3-dimethyl-7-((substituted)-2-oxo-2H-chromen-4-yl)methyl)-1H-purine-2,6(3H,7H)-dione derivatives were evaluated for their in vitro antioxidant activity by DPPH free radical-scavenging ability assay… read more here.
Keywords: synthesis coumarin; coumarin; microwave assisted; structure ... See more keywords
DNA Cleavage by Chemically Exfoliated Molybdenum Disulfide Nanosheets.
Sign Up to like & getrecommendations! Published in 2021 at "Environmental science & technology"
DOI: 10.1021/acs.est.1c00115
Abstract: Chemically exfoliated MoS2 (ce-MoS2) nanosheets have been widely used in biomedical and environmental fields. Some in vitro studies demonstrated that ce-MoS2 might induce toxicity. However, the understanding of the mechanism of potential toxicity is lacking.… read more here.
Keywords: cleavage; chemically exfoliated; mos2; cleavage chemically ... See more keywords
A Series of Spiropyrimidinetriones that Enhances DNA Cleavage Mediated by Mycobacterium tuberculosis Gyrase
Sign Up to like & getrecommendations! Published in 2023 at "ACS Infectious Diseases"
DOI: 10.1021/acsinfecdis.3c00012
Abstract: The rise in drug-resistant tuberculosis has necessitated the search for alternative antibacterial treatments. Spiropyrimidinetriones (SPTs) represent an important new class of compounds that work through gyrase, the cytotoxic target of fluoroquinolone antibacterials. The present study… read more here.
Keywords: mycobacterium tuberculosis; dna cleavage; tuberculosis; series ... See more keywords
Encounters between Cas9/dCas9 and G-Quadruplexes: Implications for Transcription Regulation and Cas9-Mediated DNA Cleavage.
Sign Up to like & getrecommendations! Published in 2021 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00067
Abstract: Using the nuclease-dead Cas9 (dCas9), we targeted in cellulo a G-rich sequence, which contains multiple potentially G-quadruplex (GQ) forming sites, within the human tyrosine hydroxylase (TH) promoter. We demonstrate that transcription can be up or… read more here.
Keywords: transcription; cas9; cas9 dcas9; sequence ... See more keywords
Bidirectional Degradation of DNA Cleavage Products Catalyzed by CRISPR/Cas9.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.7b13050
Abstract: Since the initial characterization of Streptococcus pyogenes CRISPR/Cas9 as a powerful gene-editing tool, it has been widely accepted that Cas9 generates blunt-ended DNA products by concerted cleavage of the target (tDNA) and non-target (ntDNA) strands… read more here.
Keywords: degradation; cleavage; dna; crispr cas9 ... See more keywords
Might Cu(II) binding, DNA cleavage and radical production by YadA fragments be involved in the promotion of F. nucleatum related cancers?
Sign Up to like & getrecommendations! Published in 2022 at "Dalton transactions"
DOI: 10.1039/d2dt00328g
Abstract: In many cases, human microbiota are associated with cancer progression. It was concluded that Fusobacterium nucleatum increases neoplastic changes. This bacterium is naturally present in human dental plaque. However, if it is present in the… read more here.
Keywords: might binding; radical production; production yada; cleavage radical ... See more keywords
Editor's cut: DNA cleavage by CRISPR RNA-guided nucleases Cas9 and Cas12a
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical Society Transactions"
DOI: 10.1042/bst20190563
Abstract: Discovered as an adaptive immune system of prokaryotes, CRISPR–Cas provides many promising applications. DNA-cleaving Cas enzymes like Cas9 and Cas12a, are of great interest for genome editing. The specificity of these DNA nucleases is determined… read more here.
Keywords: crispr; cas9 cas12a; dna cleavage; dna ... See more keywords
Syntheses, structural characterization, DNA-cleavage and antioxidant features of the new tetra-substituted organo-soluble non-peripherally CoII, CuII, ZnII and MgII phthalocyanines
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Coordination Chemistry"
DOI: 10.1080/00958972.2019.1648795
Abstract: Abstract This paper encompasses the synthesis, structural characterization, and evaluation of supercoiled pBR322 plasmid DNA-cleavage and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging properties of newly synthesized tetra-substituted CoII (5), CuII (6), ZnII (7) and MgII (8) phthalocyanines… read more here.
Keywords: tetra substituted; structural characterization; characterization; coii cuii ... See more keywords