Insight into an Oxidative DNA-Cleaving DNAzyme: Multiple Cofactors, the Catalytic Core Map and a Highly Efficient Variant
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DOI: 10.1016/j.isci.2020.101555
Abstract: Summary An oxidative DNA-cleaving DNAzyme (PL) employs a double-cofactor model “X/Cu2+” for catalysis. Herein, we verified that reduced nicotinamide adenine dinucleotide (NADH), flavin mononucleotide, cysteine, dithiothreitol, catechol, resorcinol, hydroquinone, phloroglucinol, o-phenylenediamine, 3,3′,5,5'-tetramethylbenzidine, and hydroxylamine acted… read more here.
Keywords: dna cleaving; oxidative dna; cleaving dnazyme; catalytic core ... See more keywords
DNAzymeBuilder, a web application for in situ generation of RNA/DNA-cleaving deoxyribozymes
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DOI: 10.1093/nar/gkac269
Abstract: Abstract Nucleic acid cleaving DNAzymes are versatile and robust catalysts that outcompete ribozymes and protein enzymes in terms of chemical stability, affordability and ease to synthesize. In spite of their attractiveness, the choice of which… read more here.
Keywords: reaction; rna dna; dnazymebuilder web; dna cleaving ... See more keywords