Optical Tweezers to Investigate the Structure and Energetics of Single-Stranded DNA-Binding Protein-DNA Complexes.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1290-3_17
Abstract: Optical tweezers enable the isolation and mechanical manipulation of individual nucleoprotein complexes. Here, we describe how to use this technique to interrogate the mechanical properties of individual protein-DNA complexes and extract information about their overall… read more here.
Keywords: optical tweezers; investigate structure; dna; protein dna ... See more keywords
Physicochemical characterization of the DNA complexes with different surfactants
Sign Up to like & getrecommendations! Published in 2021 at "Polymer"
DOI: 10.1016/j.polymer.2021.124277
Abstract: Abstract DNA complexes with cetyltrimethylammonium chloride (CTMA), benzyldimethylhexadecylammonium chloride (BAC) and hexadecylpyridinium chloride (HDP) surfactants as powder samples were studied by Fourier-Transform Middle Infrared spectroscopy, X-ray diffraction and dielectric spectroscopy methods. Complexation as electrostatic interactions… read more here.
Keywords: characterization dna; dna; dna complexes; physicochemical characterization ... See more keywords
Alchemical Free Energy Calculations for Nucleotide Mutations in Protein-DNA Complexes.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of chemical theory and computation"
DOI: 10.1021/acs.jctc.7b00849
Abstract: Nucleotide-sequence-dependent interactions between proteins and DNA are responsible for a wide range of gene regulatory functions. Accurate and generalizable methods to evaluate the strength of protein-DNA binding have long been sought. While numerous computational approaches… read more here.
Keywords: energy calculations; dna; protein dna; dna complexes ... See more keywords
Bacterial Resistance and Prostate Cancer Susceptibility Toward Metal-Ion-doped DNA Complexes.
Sign Up to like & getrecommendations! Published in 2018 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.8b17013
Abstract: DNA nanotechnology has laid a platform to construct a variety of custom-shaped nanoscale objects for functionalization of specific target materials to achieve programmability and molecular recognition. Herein, we prepared DNA nanostructures [namely, synthetic DNA rings… read more here.
Keywords: microscopy; dna; dna complexes; prostate cancer ... See more keywords
Native mass spectrometry of protein and DNA complexes prepared in non-volatile buffers.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.9b00145
Abstract: Inorganic salts and nonvolatile-buffer components affect the structure and stability of proteins, and some protein complexes are unable to maintain their function and structure without them. However, it is well known that these components cause… read more here.
Keywords: protein dna; non volatile; dna complexes; mass spectrometry ... See more keywords
A structural bioinformatics investigation on protein-DNA complexes delineates their modes of interaction.
Sign Up to like & getrecommendations! Published in 2017 at "Molecular bioSystems"
DOI: 10.1039/c7mb00071e
Abstract: The lifetimes of protein-DNA adducts are strictly related to the various protein functions. This feature must be encoded by the amino acids located at the protein-DNA interface. The large number of structurally characterized protein-DNA complexes… read more here.
Keywords: dna; protein dna; dna complexes; contact ... See more keywords
Structures of annexin A2-PS DNA complexes show dominance of hydrophobic interactions in phosphorothioate binding
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac774
Abstract: Abstract The introduction of phosphorothioate (PS) linkages to the backbone of therapeutic nucleic acids substantially increases their stability and potency. It also affects their interactions with cellular proteins, but the molecular mechanisms that underlie this… read more here.
Keywords: annexin dna; dna; complexes show; dna complexes ... See more keywords
Assembly of supramolecular DNA complexes containing both G-quadruplexes and i-motifs by enhancing the G-repeat-bearing capacity of i-motifs
Sign Up to like & getrecommendations! Published in 2017 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkw1049
Abstract: The single-step assembly of supramolecular complexes containing both i-motifs and G-quadruplexes (G4s) is demonstrated. This can be achieved because the formation of four-stranded i-motifs appears to be little affected by certain terminal residues: a five-cytosine… read more here.
Keywords: quadruplexes motifs; supramolecular dna; assembly supramolecular; dna complexes ... See more keywords
Soluble LILRA3 is aberrantly expressed in antiphospholipid syndrome (APS) and is a potential marker of thrombotic APS.
Sign Up to like & getrecommendations! Published in 2022 at "Rheumatology"
DOI: 10.1093/rheumatology/keac192
Abstract: OBJECTIVE Leucocyte immunoglobulin-like receptor A3 (LILRA3) belongs to a family of leucocyte receptors. Our previous study reported LILRA3 transcripts were markedly upregulated in neutrophils from patients with antiphospholipid syndrome (APS). We undertook this study to… read more here.
Keywords: lilra3; mpo dna; dna complexes; antiphospholipid syndrome ... See more keywords
Influence of micellar size on the structure of surfactant-DNA complexes.
Sign Up to like & getrecommendations! Published in 2022 at "Physical review. E"
DOI: 10.1103/physreve.105.064504
Abstract: We have studied the structure of complexes of the cationic surfactant dodecyltrimethylammonium bromide (DTAB) with DNA as a function of surfactant to DNA base molar ratio (R) and salt concentration. Small-angle x-ray scattering data show… read more here.
Keywords: micellar size; dna; structure; surfactant dna ... See more keywords
Interleukin-26–DNA complexes promote inflammation and dermal-epidermal separation in a modified human cryosection model of bullous pemphigoid
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2022.1013382
Abstract: Bullous pemphigoid (BP) is an autoimmune disease characterized by autoantibody-mediated activation of immune cells and subepidermal blister formation. Excess amounts of extracellular DNA are produced in BP, however, it remains unclear how extracellular DNA contributes… read more here.
Keywords: bullous pemphigoid; dna; dermal epidermal; dna complexes ... See more keywords