Computer-Aided Planning for the Verification of Large Batches of DNA Constructs.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1032-9_7
Abstract: Restriction digest analysis and Sanger sequencing are among the most commonly used techniques to check the sequence of synthetic DNA constructs. However, both require careful preparation to select restriction enzymes or DNA primers adapted to… read more here.
Keywords: large batches; dna constructs; aided planning; verification ... See more keywords
Generation of DNA Constructs Using the Golden GATEway Cloning Method.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6343-0_12
Abstract: One of the most frequently executed tasks for molecular biologists is the design and generation of complex DNA constructs. Recently, we established the Golden GATEway cloning kit for the fast and efficient generation of transgenesis… read more here.
Keywords: generation; dna constructs; golden gateway; gateway cloning ... See more keywords
In silico/In vivo analysis of high-risk papillomavirus L1 and L2 conserved sequences for development of cross-subtype prophylactic vaccine
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DOI: 10.1038/s41598-019-51679-8
Abstract: Human papillomavirus (HPV) is the most common sexually transmitted infection in the world and the main cause of cervical cancer. Nowadays, the virus-like particles (VLPs) based on L1 proteins have been considered as the best… read more here.
Keywords: dna constructs; high risk; hpv; vaccine ... See more keywords
Efficient golden gate assembly of DNA constructs for single molecule force spectroscopy and imaging
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac300
Abstract: Abstract Single-molecule techniques such as optical tweezers and fluorescence imaging are powerful tools for probing the biophysics of DNA and DNA-protein interactions. The application of these methods requires efficient approaches for creating designed DNA structures… read more here.
Keywords: single molecule; dna constructs; dna; golden gate ... See more keywords