The essential elements for the noncovalent association of two DNA ends during NHEJ synapsis
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-11507-z
Abstract: One of the most central questions about the repair of a double-strand DNA break (DSB) concerns how the two free DNA ends are brought together — a step called synapsis. Using single-molecule FRET (smFRET), we… read more here.
Keywords: two dna; dna; synapsis; dna ends ... See more keywords
Efficient N-tailing of blunt DNA ends by Moloney murine leukemia virus reverse transcriptase
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DOI: 10.1038/srep41769
Abstract: Moloney murine leukemia virus reverse transcriptase (MMLV-RT) is a widely used enzyme for cDNA synthesis. Here we show that MMLV-RT has a strong template-independent polymerase activity using blunt DNA ends as substrate that generates 3′… read more here.
Keywords: moloney murine; dna; murine leukemia; dna ends ... See more keywords
The Shieldin Complex Protects DNA Ends for 53BP1-Mediated DNA Repair.
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DOI: 10.1158/2159-8290.cd-rw2018-128
Abstract: The shieldin complex, comprised of SHLD1, SHLD2, SHLD3, and REV7, is a 53BP1 effector complex. read more here.
Keywords: shieldin complex; dna ends; shieldin; protects dna ... See more keywords
C-Terminal Extensions of Ku70 and Ku80 Differentially Influence DNA End Binding Properties
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DOI: 10.3390/ijms21186725
Abstract: The Ku70/80 heterodimer binds to DNA ends and attracts other proteins involved in the non-homologous end-joining (NHEJ) pathway of DNA double-strand break repair. We developed a novel assay to measure DNA binding and release kinetics… read more here.
Keywords: dna; end; dna ends; terminal extensions ... See more keywords