Using acetone for rapid PCR‐amplifiable DNA extraction from recalcitrant woody plant taxa
Sign Up to like & getrecommendations! Published in 2020 at "Applications in Plant Sciences"
DOI: 10.1002/aps3.11403
Abstract: Premise Quick and effective DNA extraction from plants for subsequent PCR amplification is sometimes challenging when working across diverse plant taxa that may contain a variety of inhibitory compounds. Time‐consuming methods may be needed to… read more here.
Keywords: extraction; dna; pcr; dna extraction ... See more keywords
Automated multi‐sample DNA extraction for genotyping live Xenopus embryos
Sign Up to like & getrecommendations! Published in 2022 at "Developmental Dynamics"
DOI: 10.1002/dvdy.544
Abstract: Xenopus frogs are used extensively for modeling genetic diseases owing to characteristics such as the abundance of eggs combined with their large size, allowing easy manipulation, and rapid external embryo development enabling the examination of… read more here.
Keywords: multi sample; dna extraction; automated multi; xenopus ... See more keywords
Repeated subsamples during DNA extraction reveal increased diversity estimates in DNA metabarcoding of Malaise traps
Sign Up to like & getrecommendations! Published in 2022 at "Ecology and Evolution"
DOI: 10.1002/ece3.9502
Abstract: Abstract With increased application of DNA metabarcoding in biodiversity assessment, various laboratory protocols have been optimized, and their further evaluation is subject of current research. Homogenization of bulk samples and subsequent DNA extraction from a… read more here.
Keywords: dna metabarcoding; extraction; diversity; subsamples dna ... See more keywords
A method for improving the efficiency of DNA extraction from clotted blood samples
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.22892
Abstract: The efficient and rapid extraction of high‐quality genomic DNA from clotted blood samples, which normally have a low yield and poor quality, is an important factor in genomic research. The objective of this study was… read more here.
Keywords: clotted blood; extraction; blood samples; dna extraction ... See more keywords
Evaluation of different DNA extraction methods based on steel‐bullet beating for molecular diagnosis of onychomycosis
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.24657
Abstract: Considering increased trends toward molecular methods for detection/identification of fungi causing onychomycosis, the aim of this study is comparison three DNA extraction methods based on steel‐bullet beating to extract DNA from nail. read more here.
Keywords: methods based; based steel; steel bullet; extraction methods ... See more keywords
Bovine milk microbiota: Evaluation of different DNA extraction protocols for challenging samples
Sign Up to like & getrecommendations! Published in 2022 at "MicrobiologyOpen"
DOI: 10.1002/mbo3.1275
Abstract: The use of an adequate protocol that accurately extracts microbial DNA from bovine milk samples is of importance for downstream analysis such as 16S ribosomal RNA gene sequencing. Although sequencing platforms such as Illumina are… read more here.
Keywords: dna; bovine milk; extraction; extraction protocols ... See more keywords
Development of a highly sensitive point‐of‐care test for African swine fever that combines EZ‐Fast DNA extraction with LAMP detection: Evaluation using naturally infected swine whole blood samples from Vietnam
Sign Up to like & getrecommendations! Published in 2023 at "Veterinary Medicine and Science"
DOI: 10.1002/vms3.1124
Abstract: Abstract Background While early detection and early containment are key to controlling the African swine fever (ASF) pandemic, the lack of practical testing methods for use in the field are a major barrier to achieving… read more here.
Keywords: blood samples; detection; whole blood; dna extraction ... See more keywords
Direct or DNA Extraction-Free Amplification and Quantification of Foodborne Pathogens.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9000-9_2
Abstract: The use of direct nucleic acid amplification of pathogens from food matrices has the potential to reduce time to results over DNA extraction-based approaches as well as traditional culture-based approaches. Here we describe protocols for… read more here.
Keywords: foodborne pathogens; direct dna; dna extraction; amplification ... See more keywords
Comparison of six methods of DNA extraction for the diagnosis of bovine brucellosis by real-time PCR
Sign Up to like & getrecommendations! Published in 2019 at "Archives of Microbiology"
DOI: 10.1007/s00203-019-01675-3
Abstract: Brucellosis is an infectious disease caused by bacteria of the genus Brucella, which affects domestic animals and is transmissible to humans. The objective of this study was to evaluate six methods of DNA extraction directly… read more here.
Keywords: extraction; methods dna; comparison six; six methods ... See more keywords
DNA extraction on bio-chip: history and preeminence over conventional and solid-phase extraction methods
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8493-0
Abstract: This review covers a developmental progression on early to modern taxonomy at cellular level following the advent of electron microscopy and the advancement in deoxyribonucleic acid (DNA) extraction for expatiation of biological classification at DNA… read more here.
Keywords: extraction; solid phase; dna extraction; phase extraction ... See more keywords
Impact of DNA extraction, sample dilution, and reagent contamination on 16S rRNA gene sequencing of human feces
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8583-z
Abstract: Culture-independent methods have granted the possibility to study microbial diversity in great detail, but technical issues pose a threat to the accuracy of new findings. Biases introduced during DNA extraction can result in erroneous representations… read more here.
Keywords: sample; extraction; dna extraction; reagent contamination ... See more keywords