Using acetone for rapid PCR‐amplifiable DNA extraction from recalcitrant woody plant taxa
Sign Up to like & getrecommendations! Published in 2020 at "Applications in Plant Sciences"
DOI: 10.1002/aps3.11403
Abstract: Premise Quick and effective DNA extraction from plants for subsequent PCR amplification is sometimes challenging when working across diverse plant taxa that may contain a variety of inhibitory compounds. Time‐consuming methods may be needed to… read more here.
Keywords: extraction; dna; pcr; dna extraction ... See more keywords
Optimizing DNA extraction protocols for bryophytes: Insights from Orthotrichaceae
Sign Up to like & getrecommendations! Published in 2025 at "Applications in Plant Sciences"
DOI: 10.1002/aps3.70028
Abstract: Abstract Premise Extracting high‐quality DNA from challenging plant tissues can be hindered by high levels of phenolics, carbohydrates, and other compounds that bind to or coprecipitate with DNA. Bryophytes, due to their small size, tendency… read more here.
Keywords: protocols bryophytes; extraction protocols; dna extraction; extraction ... See more keywords
Automated multi‐sample DNA extraction for genotyping live Xenopus embryos
Sign Up to like & getrecommendations! Published in 2022 at "Developmental Dynamics"
DOI: 10.1002/dvdy.544
Abstract: Xenopus frogs are used extensively for modeling genetic diseases owing to characteristics such as the abundance of eggs combined with their large size, allowing easy manipulation, and rapid external embryo development enabling the examination of… read more here.
Keywords: multi sample; dna extraction; automated multi; xenopus ... See more keywords
Optimising recovery of DNA from minimally invasive sampling methods: Efficacy of buccal swabs, preservation strategy and DNA extraction approaches for amphibian studies
Sign Up to like & getrecommendations! Published in 2024 at "Ecology and Evolution"
DOI: 10.1002/ece3.70294
Abstract: Abstract Studies in evolution, ecology and conservation are increasingly based on genetic and genomic data. With increased focus on molecular approaches, ethical concerns about destructive or more invasive techniques need to be considered, with a… read more here.
Keywords: buccal swabs; dna extraction; extraction; ecology ... See more keywords
Comparison of Fecal DNA Extraction Kits for the Giant Panda (Ailuropoda melanoleuca) by Short Tandem Repeat Genotype Analysis
Sign Up to like & getrecommendations! Published in 2025 at "Ecology and Evolution"
DOI: 10.1002/ece3.71242
Abstract: ABSTRACT Genetic analysis of short tandem repeat (STR) loci using noninvasive fecal samples is currently the most widely used method in genetic surveys of giant pandas ( Ailuropoda melanoleuca ). However, low‐quality fecal DNA obtained… read more here.
Keywords: fecal dna; dna extraction; extraction; kit kit ... See more keywords
Repeated subsamples during DNA extraction reveal increased diversity estimates in DNA metabarcoding of Malaise traps
Sign Up to like & getrecommendations! Published in 2022 at "Ecology and Evolution"
DOI: 10.1002/ece3.9502
Abstract: Abstract With increased application of DNA metabarcoding in biodiversity assessment, various laboratory protocols have been optimized, and their further evaluation is subject of current research. Homogenization of bulk samples and subsequent DNA extraction from a… read more here.
Keywords: dna metabarcoding; extraction; diversity; subsamples dna ... See more keywords
A method for improving the efficiency of DNA extraction from clotted blood samples
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.22892
Abstract: The efficient and rapid extraction of high‐quality genomic DNA from clotted blood samples, which normally have a low yield and poor quality, is an important factor in genomic research. The objective of this study was… read more here.
Keywords: clotted blood; extraction; blood samples; dna extraction ... See more keywords
Evaluation of different DNA extraction methods based on steel‐bullet beating for molecular diagnosis of onychomycosis
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.24657
Abstract: Considering increased trends toward molecular methods for detection/identification of fungi causing onychomycosis, the aim of this study is comparison three DNA extraction methods based on steel‐bullet beating to extract DNA from nail. read more here.
Keywords: methods based; based steel; steel bullet; extraction methods ... See more keywords
Bovine milk microbiota: Evaluation of different DNA extraction protocols for challenging samples
Sign Up to like & getrecommendations! Published in 2022 at "MicrobiologyOpen"
DOI: 10.1002/mbo3.1275
Abstract: The use of an adequate protocol that accurately extracts microbial DNA from bovine milk samples is of importance for downstream analysis such as 16S ribosomal RNA gene sequencing. Although sequencing platforms such as Illumina are… read more here.
Keywords: dna; bovine milk; extraction; extraction protocols ... See more keywords
Evaluation of DNA Extraction Methods for Microbial Community Profiling in Deadwood Decomposition
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DOI: 10.1002/mbo3.70007
Abstract: As technologies advance alongside metabarcoding and metagenomic resources, particularly for larger fungal genomes, DNA extraction methods must be optimized to meet higher thresholds, especially from complex environmental substrates. This study focused on extracting fungal genomic… read more here.
Keywords: dna extraction; wood; extraction methods; extraction ... See more keywords
Development of a highly sensitive point‐of‐care test for African swine fever that combines EZ‐Fast DNA extraction with LAMP detection: Evaluation using naturally infected swine whole blood samples from Vietnam
Sign Up to like & getrecommendations! Published in 2023 at "Veterinary Medicine and Science"
DOI: 10.1002/vms3.1124
Abstract: Abstract Background While early detection and early containment are key to controlling the African swine fever (ASF) pandemic, the lack of practical testing methods for use in the field are a major barrier to achieving… read more here.
Keywords: blood samples; detection; whole blood; dna extraction ... See more keywords