The discovery of potent immunostimulatory CpG-ODNs widely distributed in bacterial genomes
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Microbiology"
DOI: 10.1007/s12275-020-9289-y
Abstract: Oligodeoxynucleotides containing unmethylated CpG dinucleotides (CpG-ODN) can be specifically recognized by Toll-like receptor 9 (TLR9), provoking innate immune responses. Designed according to this structural feature, many synthetic phosphorothioate CpG-ODNs successfully activate macrophages. However, it is… read more here.
Keywords: dna; dna fragments; stimulatory cpg; cpg odns ... See more keywords
Identifying Transcription Factors That Prefer Binding to Methylated DNA Using Reduced G-Gap Dipeptide Composition
Sign Up to like & getrecommendations! Published in 2022 at "ACS Omega"
DOI: 10.1021/acsomega.2c03696
Abstract: Transcription factors (TFs) play an important role in gene expression and regulation of 3D genome conformation. TFs have ability to bind to specific DNA fragments called enhancers and promoters. Some TFs bind to promoter DNA… read more here.
Keywords: dna; transcription; methylated dna; tfs ... See more keywords
Genomic Iterative Replacements of Large Synthetic DNA Fragments in Corynebacterium glutamicum.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00644
Abstract: Synthetic genomics will advance our understanding of life and allow us to rebuild the genomes of industrial microorganisms for enhancing performances. Corynebacterium glutamicum, a Gram-positive bacterium, is an important industrial workhorse. However, its genome synthesis… read more here.
Keywords: corynebacterium glutamicum; genomic iterative; dna fragments; glutamicum ... See more keywords
The mitochondrial DNA polymerase gamma degrades linear DNA fragments precluding the formation of deletions
Sign Up to like & getrecommendations! Published in 2018 at "Nature Communications"
DOI: 10.1038/s41467-018-04895-1
Abstract: Double-strand breaks in the mitochondrial DNA (mtDNA) result in the formation of linear fragments that are rapidly degraded. However, the identity of the nuclease(s) performing this function is not known. We found that the exonuclease… read more here.
Keywords: dna; dna fragments; formation; polymerase gamma ... See more keywords
An accurate algorithm for the detection of DNA fragments from dilution pool sequencing experiments
Sign Up to like & getrecommendations! Published in 2018 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btx436
Abstract: Motivation The short read lengths of current high-throughput sequencing technologies limit the ability to recover long-range haplotype information. Dilution pool methods for preparing DNA sequencing libraries from high molecular weight DNA fragments enable the recovery… read more here.
Keywords: pool; dilution pool; dna fragments; fragments dilution ... See more keywords
NAFLD between genes and environment: what drives fibrogenesis?
Sign Up to like & getrecommendations! Published in 2020 at "Gut"
DOI: 10.1136/gutjnl-2020-321964
Abstract: ABSTRACT DNA fragments were amplified by PCR from all tested strains of Aeromonas hydrophila, A. caviae, and A. sobria with primers designed based on sequence alignment of all lipase, phospholipase C, and phospholipase A1 genes… read more here.
Keywords: amplified dna; dna; dna fragments; caviae sobria ... See more keywords
Allergen-stimulated secretion of DNA fragments by the airway epithelium enhances type 2 immune responses
Sign Up to like & getrecommendations! Published in 2023 at "Physiology"
DOI: 10.1152/physiol.2023.38.s1.5730041
Abstract: Exposure of human bronchial epithelial cells (hBECs) and mouse airways to allergens from the fungus Alternaria alternata triggers the release of alarmin signaling molecules including ATP, IL-33 and DNA fragments. The goal of this study… read more here.
Keywords: dna; dna release; type immune; dna fragments ... See more keywords
Expanding role of deoxyribonucleic acid-sensing mechanism in the development of lifestyle-related diseases
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cardiovascular Medicine"
DOI: 10.3389/fcvm.2022.881181
Abstract: In lifestyle-related diseases, such as cardiovascular, metabolic, respiratory, and kidney diseases, chronic inflammation plays a causal role in their pathogenesis; however, underlying mechanisms of sterile chronic inflammation are not well-understood. Previous studies have confirmed the… read more here.
Keywords: lifestyle related; dna; role; dna fragments ... See more keywords
Detection and Validation of Circular DNA Fragments Using Nanopore Sequencing
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Genetics"
DOI: 10.3389/fgene.2022.867018
Abstract: Occurrence of extra-chromosomal circular DNA is a phenomenon frequently observed in tumor cells, and the presence of such DNA has been recognized as a marker of adverse outcome across cancer types. We here describe a… read more here.
Keywords: circular dna; dna; dna fragments; detection ... See more keywords
A Python script to design primers for overlap extension PCR to ligate two DNA fragments
Sign Up to like & getrecommendations! Published in 2022 at "PeerJ"
DOI: 10.7717/peerj.14283
Abstract: Ligating two or more DNA fragments is a regular operation for the subcloning and the engineering of vectors. The overlap extension PCR serves as a straightforward method to solve this issue. However, it takes a… read more here.
Keywords: overlap extension; python script; dna fragments; design ... See more keywords